We describe here a fatty acid–binding protein (FABP) isolated and purified from the parasitic protozoon Giardia lamblia. The protein has a molecular mass of 8 kDa and an isoelectric point of 4.96. A Scatchard analysis of the data at equilibrium revealed a dissociation constant of 3.12 × 10−8 M when the labeled oleic acid was displaced by a 10-fold greater concentration of unlabeled oleic acid. Testosterone, sodium desoxycholate, taurocholate, metronidazol, and α-tocopherol, together with butyric, arachidonic, palmitic, retinoic, and glycocholic acids, were also bound to the protein. Assays with polyclonal antibodies revealed that the protein is located in the ventral disk and also appears in the dorsal membrane, the cytoplasm, and in the vicinity of the lipid vacuoles.
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April 2005
MOLECULAR-CELL BIOLOGY|
April 01 2005
BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA Available to Purchase
S. M T. Hassan;
S. M T. Hassan
Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, E-18071 Granada, Spain. [email protected]
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M. Maache;
M. Maache
Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, E-18071 Granada, Spain. [email protected]
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R. Díaz de la Guardia;
R. Díaz de la Guardia
Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, E-18071 Granada, Spain. [email protected]
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O. M. Córdova;
O. M. Córdova
Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, E-18071 Granada, Spain. [email protected]
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J. R. Gil García;
J. R. Gil García
Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, E-18071 Granada, Spain. [email protected]
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M. Galiana;
M. Galiana
Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, E-18071 Granada, Spain. [email protected]
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D. Acuña Castroviejo;
D. Acuña Castroviejo
Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, E-18071 Granada, Spain. [email protected]
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M. Martins;
M. Martins
Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, E-18071 Granada, Spain. [email protected]
* Fundação de Medicina Tropical-FMT/IMT-AM, Gerência de Parasitologia, Manaus-AM CEP: 69040 000, Brazil
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Antonio Osuna
Antonio Osuna
Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, E-18071 Granada, Spain. [email protected]
† To whom correspondence should be addressed
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J Parasitol (2005) 91 (2): 284–292.
Citation
S. M T. Hassan, M. Maache, R. Díaz de la Guardia, O. M. Córdova, J. R. Gil García, M. Galiana, D. Acuña Castroviejo, M. Martins, Antonio Osuna; BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA. J Parasitol 1 April 2005; 91 (2): 284–292. doi: https://doi.org/10.1645/GE-3352
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