Severe malaria is associated with the failure of host defenses to control parasite replication, with the excessive secretion of proinflammatory cytokines such as tumor necrosis factor–α (TNF-α), and with the sequestration of parasitized erythrocytes (PEs) in the microcirculation of vital organs. The scavenger receptor CD36, known as a major sequestration receptor, has also been identified as an important factor in mediating nonopsonic phagocytosis of PEs by monocytes and macrophages. The specific consequence of this phagocytosis is a decrease in parasite-induced TNF-α secretion. We evaluated the variations in CD36 level and in lipopolysaccharide (LPS)-induced TNF-α production in monocytes from Plasmodium falciparum–infected patients and in vitro in the presence of PEs. Both the monocytes from infected patients and from in vitro culture showed a decrease of CD36 expression and a reduced production of TNF-α induced by LPS. Using incubation assays with no contact between monocytes and PEs, or in the presence of a soluble supernatant obtained from the incubation of monocytes and PEs, this study shows that decreased CD36 expression was posttranscriptional and not directly related to PEs phagocytosis. In addition, these culture models suggest that the reduced capacity of TNF-α production occurred in 2 phases. The early phase (24 hr) appeared to be CD36 dependent and the second phase (48 hr) was due to a soluble factor produced by PEs. These observations suggest that the control of the TNF-α production in malaria by monocytes was not entirely dependent on the phagocytosis of PEs by CD36 and that soluble factors produced by PEs could play a role in this process.
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April 2005
SYSTEMATICS-PHYLOGENETICS|
April 01 2005
EX VIVO AND IN VITRO IMPAIRMENT OF CD36 EXPRESSION AND TUMOR NECROSIS FACTOR-α PRODUCTION IN HUMAN MONOCYTES IN RESPONSE TO PLASMODIUM FALCIPARUM–PARASITIZED ERYTHROCYTES Available to Purchase
A. Berry;
A. Berry
Service de Parasitologie Mycologie, CHU Rangueil, TSA 50032, 31059 Toulouse Cedex 9, France. [email protected]
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G. Chene;
G. Chene
Service de Parasitologie Mycologie, CHU Rangueil, TSA 50032, 31059 Toulouse Cedex 9, France. [email protected]
* Macrophages, Mediateurs de l'Inflammation et Interactions Cellulaires, UPRES EA 2405 INSERM IFR 31, CHU Rangueil, 31059 Toulouse, France
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F. Benoit-Vical;
F. Benoit-Vical
Service de Parasitologie Mycologie, CHU Rangueil, TSA 50032, 31059 Toulouse Cedex 9, France. [email protected]
† Laboratoire de Chimie de Coordination du CNRS, UPR CNRS 8241, 31077 Toulouse, France
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J. C. Lepert;
J. C. Lepert
Service de Parasitologie Mycologie, CHU Rangueil, TSA 50032, 31059 Toulouse Cedex 9, France. [email protected]
* Macrophages, Mediateurs de l'Inflammation et Interactions Cellulaires, UPRES EA 2405 INSERM IFR 31, CHU Rangueil, 31059 Toulouse, France
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J. Bernad;
J. Bernad
Service de Parasitologie Mycologie, CHU Rangueil, TSA 50032, 31059 Toulouse Cedex 9, France. [email protected]
* Macrophages, Mediateurs de l'Inflammation et Interactions Cellulaires, UPRES EA 2405 INSERM IFR 31, CHU Rangueil, 31059 Toulouse, France
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B. Marchou;
B. Marchou
Service de Parasitologie Mycologie, CHU Rangueil, TSA 50032, 31059 Toulouse Cedex 9, France. [email protected]
‡ Service des Maladies Infectieuses et Tropicales, CHU Purpan, 31059 Toulouse, France
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J. P. Séguéla;
J. P. Séguéla
Service de Parasitologie Mycologie, CHU Rangueil, TSA 50032, 31059 Toulouse Cedex 9, France. [email protected]
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J. F. Magnaval;
J. F. Magnaval
Service de Parasitologie Mycologie, CHU Rangueil, TSA 50032, 31059 Toulouse Cedex 9, France. [email protected]
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B. Pipy
B. Pipy
Service de Parasitologie Mycologie, CHU Rangueil, TSA 50032, 31059 Toulouse Cedex 9, France. [email protected]
* Macrophages, Mediateurs de l'Inflammation et Interactions Cellulaires, UPRES EA 2405 INSERM IFR 31, CHU Rangueil, 31059 Toulouse, France
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J Parasitol (2005) 91 (2): 316–322.
Citation
A. Berry, G. Chene, F. Benoit-Vical, J. C. Lepert, J. Bernad, B. Marchou, J. P. Séguéla, J. F. Magnaval, B. Pipy; EX VIVO AND IN VITRO IMPAIRMENT OF CD36 EXPRESSION AND TUMOR NECROSIS FACTOR-α PRODUCTION IN HUMAN MONOCYTES IN RESPONSE TO PLASMODIUM FALCIPARUM–PARASITIZED ERYTHROCYTES. J Parasitol 1 April 2005; 91 (2): 316–322. doi: https://doi.org/10.1645/GE-346R
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