Mechanisms of host immunosuppression after infection with Toxoplasma gondii are unclear. This study was performed to observe cytokine and immunoglobulin secretions by murine splenic lymphocytes infected in vitro with live, nonreplicating (irradiated) RH tachyzoites on stimulation with concanavalin A (Con A) or lipopolysaccharide (LPS). For lymphocyte cultivation, 3 groups were prepared: coculture with live nonirradiated tachyzoites separated by a transwell (group T), live irradiated tachyzoites without a transwell (group R), and no tachyzoites (group C). Compared with group T, groups R and C, on stimulation with Con A, revealed significantly (P < 0.05) lower levels of interleukin-2 (IL-2) and IFN-γ, but not IL-10. The levels of IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM were also significantly (P < 0.05) lower in groups R and C than in group T after stimulation with LPS. The results suggest that intracellular infection of murine splenic lymphocytes with T. gondii tachyzoites could impair their capacity to produce cytokine and immunoglobulin secretions.
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April 2005
RESEARCH NOTES|
April 01 2005
Suppressed Cytokine and Immunoglobulin Secretions by Murine Splenic Lymphocytes Infected In Vitro with Toxoplasma gondii Tachyzoites
S-M. Guk;
S-M. Guk
aDepartment of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea
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J. Kook;
J. Kook
aDepartment of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea
bDepartment of Biochemical Engineering, Yanbian University of Science and Technology, Yanji City, Jilin Province 133000, P.R. China
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Y-H. Jeon;
Y-H. Jeon
aDepartment of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea
cDepartment of Pediatrics, Inha University College of Medicine, Inchon 400-711, Korea
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J-H. Choi;
J-H. Choi
aDepartment of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea
dDepartment of Pediatrics, Seoul National University Children's Hospital, Seoul 110-744, Korea
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E-T. Han;
E-T. Han
aDepartment of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea
eDepartment of Parasitology, Kangwon National University College of Medicine, Chunchon 200-701, Korea
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E-H. Shin;
E-H. Shin
aDepartment of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea
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J-Y. Chai
J-Y. Chai
aDepartment of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea
fTo whom correspondence should be addressed. [email protected]
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J Parasitol (2005) 91 (2): 467–470.
Citation
S-M. Guk, J. Kook, Y-H. Jeon, J-H. Choi, E-T. Han, E-H. Shin, J-Y. Chai; Suppressed Cytokine and Immunoglobulin Secretions by Murine Splenic Lymphocytes Infected In Vitro with Toxoplasma gondii Tachyzoites. J Parasitol 1 April 2005; 91 (2): 467–470. doi: https://doi.org/10.1645/GE-404R
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