Neosporosis is an important cause of pregnancy loss in cattle worldwide. The objective of the present study was to identify Neospora caninum antigens as vaccine candidates using antigen-specific, short-term CD4+ T cells established from N. caninum–immunized and –challenged cows. Whole N. caninum tachyzoite lysate was separated into 6 fractions by DEAE anion-exchange chromatography using high-pressure liquid chromatography (HPLC). The CD4+ T-cell proliferation assay results indicated that antigenic activity was associated with proteins from HPLC fractions 4–6, with fraction 5 exhibiting the highest antigenic activity. Also, SDS-PAGE analysis revealed a 16-kDa protein in fractions 4–6 that was recognized by anti–N. caninum antibodies. This 16-kDa protein was absent in other fractions, and it may be a target of a T-cell response in cattle. Further identification of immunogenic proteins of N. caninum may facilitate development of subunit vaccines against neosporosis.
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June 2005
IMMUNOLOGY|
June 01 2005
NEOSPORA CANINUM ANTIGENS DEFINED BY ANTIGEN-DEPENDENT BOVINE CD4+ T CELLS Available to Purchase
Wenbin Tuo;
Wenbin Tuo
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705. [email protected]
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Raymond H. Fetterer;
Raymond H. Fetterer
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705. [email protected]
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William C. Davis;
William C. Davis
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705. [email protected]
* Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164
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Mark C. Jenkins;
Mark C. Jenkins
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705. [email protected]
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J. P. Dubey
J. P. Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705. [email protected]
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J Parasitol (2005) 91 (3): 564–568.
Citation
Wenbin Tuo, Raymond H. Fetterer, William C. Davis, Mark C. Jenkins, J. P. Dubey; NEOSPORA CANINUM ANTIGENS DEFINED BY ANTIGEN-DEPENDENT BOVINE CD4+ T CELLS. J Parasitol 1 June 2005; 91 (3): 564–568. doi: https://doi.org/10.1645/GE-386R
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