Neosporosis is an important cause of pregnancy loss in cattle worldwide. The objective of the present study was to identify Neospora caninum antigens as vaccine candidates using antigen-specific, short-term CD4+ T cells established from N. caninum–immunized and –challenged cows. Whole N. caninum tachyzoite lysate was separated into 6 fractions by DEAE anion-exchange chromatography using high-pressure liquid chromatography (HPLC). The CD4+ T-cell proliferation assay results indicated that antigenic activity was associated with proteins from HPLC fractions 4–6, with fraction 5 exhibiting the highest antigenic activity. Also, SDS-PAGE analysis revealed a 16-kDa protein in fractions 4–6 that was recognized by anti–N. caninum antibodies. This 16-kDa protein was absent in other fractions, and it may be a target of a T-cell response in cattle. Further identification of immunogenic proteins of N. caninum may facilitate development of subunit vaccines against neosporosis.
Skip Nav Destination
Article navigation
June 2005
IMMUNOLOGY|
June 01 2005
NEOSPORA CANINUM ANTIGENS DEFINED BY ANTIGEN-DEPENDENT BOVINE CD4+ T CELLS Available to Purchase
Wenbin Tuo;
Wenbin Tuo
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705. [email protected]
Search for other works by this author on:
Raymond H. Fetterer;
Raymond H. Fetterer
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705. [email protected]
Search for other works by this author on:
William C. Davis;
William C. Davis
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705. [email protected]
* Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164
Search for other works by this author on:
Mark C. Jenkins;
Mark C. Jenkins
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705. [email protected]
Search for other works by this author on:
J. P. Dubey
J. P. Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705. [email protected]
Search for other works by this author on:
J Parasitol (2005) 91 (3): 564–568.
Citation
Wenbin Tuo, Raymond H. Fetterer, William C. Davis, Mark C. Jenkins, J. P. Dubey; NEOSPORA CANINUM ANTIGENS DEFINED BY ANTIGEN-DEPENDENT BOVINE CD4+ T CELLS. J Parasitol 1 June 2005; 91 (3): 564–568. doi: https://doi.org/10.1645/GE-386R
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
HAPLOTYPE DIVERSITY IN ENDOSYMBIOTIC BACTERIA FOLLOWING A HOST SWITCH BY PARASITIC LICE
Bret M. Boyd, Niyomi House, Ariel C. Toloza, David L. Reed
DESCRIPTION, REDESCRIPTION, AND LIFE CYCLES OF CLOACITREMA KURISI N. SP. AND CLOACITREMA MICHIGANENSIS (TREMATODA: DIGENEA: PHILOPHTHALMIDAE) FROM THE CALIFORNIA HORN SNAIL, CERITHIDEOPSIS CALIFORNICA (GASTROPODA: POTAMIDIDAE)
Alexandria P. Nelson, Daniel C. G. Metz, Ryan F. Hechinger
A NEW SPECIES OF HALIPEGUS (TREMATODA: DEROGENIDAE: HALIPEGINAE) IN LITHOBATES SPECTABILIS (ANURA: RANIDAE) FROM RESERVA DE LA BIOSFERA BARRANCA DE METZTITLAN, HIDALGO, MEXICO
Edgar S. Ramírez-Cruz, Griselda Pulido-Flores, Scott Monks, Elizabeth A. Martínez-Salazar
A SYSTEMATIC REVISION OF KROYERIA (COPEPODA: SIPHONOSTOMATOIDA): FIVE NEW SPECIES, 11 REDESCRIPTIONS, AND A MORPHOLOGICAL PHYLOGENETIC ANALYSIS OF THE GENUS
Gregory B. Deets, Geoffrey A. Boxshall, James P. Bernot
MOLECULAR SURVEY OF HAEMOSPORIDIAN PARASITES IN HAWKS, FALCONS, AND OWLS (ACCIPITRIFORMES, FALCONIFORMES, STRIGIFORMES) FROM MINNESOTA AND NORTH DAKOTA, WITH REMARKS ON THE PHYLOGENETIC RELATIONSHIPS OF HAEMOSPORIDIANS IN NORTH AMERICAN RAPTORS
Jeffrey A. Bell, Timothy G. Driscoll, Tyler J. Achatz, Jakson R. Martens, Jefferson A. Vaughan