Molecular identification of dorsal-spined larvae (DSL) from fecal samples indicates that the protostrongylid parasite Parelaphostrongylus odocoilei occupies a broader geographic range in western North America than has been previously reported. We analyzed 2,124 fecal samples at 29 locations from thinhorn sheep (Ovis dalli dalli and O. d. stonei), bighorn sheep (Ovis canadensis canadensis and O. c. californiana), mountain goats (Oreamnos americanus), woodland caribou (Rangifer tarandus caribou), mule deer (Odocoileus hemionus hemionus), and black-tailed deer (O. h. columbianus). The DSL were recovered from populations of thinhorn sheep south, but not north, of the Arctic Circle, and they were not recovered from any of the bighorn sheep populations that we examined. In total, DSL were recovered from 20 locations in the United States and Canada (Alaska, Yukon Territory, Northwest Territories, British Columbia, Alberta, and California). The DSL were identified as P. odocoilei by comparing sequences of the second internal transcribed spacer (ITS2) region of ribosomal RNA among 9 protostrongylid species validated by adult comparative morphology. The ITS2 sequences were markedly different between Parelaphostrongylus and other protostrongylid genera. Smaller fixed differences served as diagnostic markers for the 3 species of Parelaphostrongylus. The ITS2 sequences (n = 60) of P. odocoilei were strongly conserved across its broad geographic range from California to Alaska. Polymorphism at 5 nucleotide positions was consistent with multiple copies of the ITS2 within individual specimens of P. odocoilei. This work combines extensive fecal surveys, comparative morphology, and molecular diagnostic techniques to describe comprehensively the host associations and geographic distribution of a parasitic helminth.
Skip Nav Destination
Article navigation
June 2005
LIFE CYCLES-SURVEY|
June 01 2005
GEOGRAPHIC DISTRIBUTION OF THE MUSCLE-DWELLING NEMATODE PARELAPHOSTRONGYLUS ODOCOILEI IN NORTH AMERICA, USING MOLECULAR IDENTIFICATION OF FIRST-STAGE LARVAE Available to Purchase
Emily J. Jenkins;
Emily J. Jenkins
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
Search for other works by this author on:
Greg D. Appleyard;
Greg D. Appleyard
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
Search for other works by this author on:
Eric P. Hoberg;
Eric P. Hoberg
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
* U.S. National Parasite Collection and Animal Parasitic Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, 20705
Search for other works by this author on:
Benjamin M. Rosenthal;
Benjamin M. Rosenthal
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
* U.S. National Parasite Collection and Animal Parasitic Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, 20705
Search for other works by this author on:
Susan J. Kutz;
Susan J. Kutz
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
Search for other works by this author on:
Alasdair M. Veitch;
Alasdair M. Veitch
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
† Department of Resources, Wildlife, and Economic Development, Government of the Northwest Territories, Norman Wells, Northwest Territories X0E 0V0, Canada
Search for other works by this author on:
Helen M. Schwantje;
Helen M. Schwantje
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
‡ Biodiversity Branch, Ministry of Water, Land, and Air Protection, Victoria, British Columbia V8W 9M1, Canada
Search for other works by this author on:
Brett T. Elkin;
Brett T. Elkin
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
§ Department of Resources, Wildlife, and Economic Development, Government of the Northwest Territories, Yellowknife, Northwest Territories X1A 3S8, Canada
Search for other works by this author on:
Lydden Polley
Lydden Polley
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
Search for other works by this author on:
J Parasitol (2005) 91 (3): 574–584.
Citation
Emily J. Jenkins, Greg D. Appleyard, Eric P. Hoberg, Benjamin M. Rosenthal, Susan J. Kutz, Alasdair M. Veitch, Helen M. Schwantje, Brett T. Elkin, Lydden Polley; GEOGRAPHIC DISTRIBUTION OF THE MUSCLE-DWELLING NEMATODE PARELAPHOSTRONGYLUS ODOCOILEI IN NORTH AMERICA, USING MOLECULAR IDENTIFICATION OF FIRST-STAGE LARVAE. J Parasitol 1 June 2005; 91 (3): 574–584. doi: https://doi.org/10.1645/GE-413R
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
HAPLOTYPE DIVERSITY IN ENDOSYMBIOTIC BACTERIA FOLLOWING A HOST SWITCH BY PARASITIC LICE
Bret M. Boyd, Niyomi House, Ariel C. Toloza, David L. Reed
DESCRIPTION, REDESCRIPTION, AND LIFE CYCLES OF CLOACITREMA KURISI N. SP. AND CLOACITREMA MICHIGANENSIS (TREMATODA: DIGENEA: PHILOPHTHALMIDAE) FROM THE CALIFORNIA HORN SNAIL, CERITHIDEOPSIS CALIFORNICA (GASTROPODA: POTAMIDIDAE)
Alexandria P. Nelson, Daniel C. G. Metz, Ryan F. Hechinger
A NEW SPECIES OF HALIPEGUS (TREMATODA: DEROGENIDAE: HALIPEGINAE) IN LITHOBATES SPECTABILIS (ANURA: RANIDAE) FROM RESERVA DE LA BIOSFERA BARRANCA DE METZTITLAN, HIDALGO, MEXICO
Edgar S. Ramírez-Cruz, Griselda Pulido-Flores, Scott Monks, Elizabeth A. Martínez-Salazar
A SYSTEMATIC REVISION OF KROYERIA (COPEPODA: SIPHONOSTOMATOIDA): FIVE NEW SPECIES, 11 REDESCRIPTIONS, AND A MORPHOLOGICAL PHYLOGENETIC ANALYSIS OF THE GENUS
Gregory B. Deets, Geoffrey A. Boxshall, James P. Bernot
MOLECULAR SURVEY OF HAEMOSPORIDIAN PARASITES IN HAWKS, FALCONS, AND OWLS (ACCIPITRIFORMES, FALCONIFORMES, STRIGIFORMES) FROM MINNESOTA AND NORTH DAKOTA, WITH REMARKS ON THE PHYLOGENETIC RELATIONSHIPS OF HAEMOSPORIDIANS IN NORTH AMERICAN RAPTORS
Jeffrey A. Bell, Timothy G. Driscoll, Tyler J. Achatz, Jakson R. Martens, Jefferson A. Vaughan