Molecular identification of dorsal-spined larvae (DSL) from fecal samples indicates that the protostrongylid parasite Parelaphostrongylus odocoilei occupies a broader geographic range in western North America than has been previously reported. We analyzed 2,124 fecal samples at 29 locations from thinhorn sheep (Ovis dalli dalli and O. d. stonei), bighorn sheep (Ovis canadensis canadensis and O. c. californiana), mountain goats (Oreamnos americanus), woodland caribou (Rangifer tarandus caribou), mule deer (Odocoileus hemionus hemionus), and black-tailed deer (O. h. columbianus). The DSL were recovered from populations of thinhorn sheep south, but not north, of the Arctic Circle, and they were not recovered from any of the bighorn sheep populations that we examined. In total, DSL were recovered from 20 locations in the United States and Canada (Alaska, Yukon Territory, Northwest Territories, British Columbia, Alberta, and California). The DSL were identified as P. odocoilei by comparing sequences of the second internal transcribed spacer (ITS2) region of ribosomal RNA among 9 protostrongylid species validated by adult comparative morphology. The ITS2 sequences were markedly different between Parelaphostrongylus and other protostrongylid genera. Smaller fixed differences served as diagnostic markers for the 3 species of Parelaphostrongylus. The ITS2 sequences (n = 60) of P. odocoilei were strongly conserved across its broad geographic range from California to Alaska. Polymorphism at 5 nucleotide positions was consistent with multiple copies of the ITS2 within individual specimens of P. odocoilei. This work combines extensive fecal surveys, comparative morphology, and molecular diagnostic techniques to describe comprehensively the host associations and geographic distribution of a parasitic helminth.
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June 2005
LIFE CYCLES-SURVEY|
June 01 2005
GEOGRAPHIC DISTRIBUTION OF THE MUSCLE-DWELLING NEMATODE PARELAPHOSTRONGYLUS ODOCOILEI IN NORTH AMERICA, USING MOLECULAR IDENTIFICATION OF FIRST-STAGE LARVAE
Emily J. Jenkins;
Emily J. Jenkins
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
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Greg D. Appleyard;
Greg D. Appleyard
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
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Eric P. Hoberg;
Eric P. Hoberg
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
* U.S. National Parasite Collection and Animal Parasitic Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, 20705
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Benjamin M. Rosenthal;
Benjamin M. Rosenthal
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
* U.S. National Parasite Collection and Animal Parasitic Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, 20705
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Susan J. Kutz;
Susan J. Kutz
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
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Alasdair M. Veitch;
Alasdair M. Veitch
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
† Department of Resources, Wildlife, and Economic Development, Government of the Northwest Territories, Norman Wells, Northwest Territories X0E 0V0, Canada
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Helen M. Schwantje;
Helen M. Schwantje
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
‡ Biodiversity Branch, Ministry of Water, Land, and Air Protection, Victoria, British Columbia V8W 9M1, Canada
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Brett T. Elkin;
Brett T. Elkin
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
§ Department of Resources, Wildlife, and Economic Development, Government of the Northwest Territories, Yellowknife, Northwest Territories X1A 3S8, Canada
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Lydden Polley
Lydden Polley
Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada, S7N 5B4. [email protected]
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J Parasitol (2005) 91 (3): 574–584.
Citation
Emily J. Jenkins, Greg D. Appleyard, Eric P. Hoberg, Benjamin M. Rosenthal, Susan J. Kutz, Alasdair M. Veitch, Helen M. Schwantje, Brett T. Elkin, Lydden Polley; GEOGRAPHIC DISTRIBUTION OF THE MUSCLE-DWELLING NEMATODE PARELAPHOSTRONGYLUS ODOCOILEI IN NORTH AMERICA, USING MOLECULAR IDENTIFICATION OF FIRST-STAGE LARVAE. J Parasitol 1 June 2005; 91 (3): 574–584. doi: https://doi.org/10.1645/GE-413R
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