Genetic Characterization of Cryptosporidium Isolates From Ringed Seals (Phoca hispida) in Northern Quebec, Canada

Santín, Mónica; Dixon, Brent R.; Fayer, Ronald

doi:10.1645/GE-3438RN

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RESEARCH NOTES| June 01 2005

Genetic Characterization of Cryptosporidium Isolates From Ringed Seals (Phoca hispida) in Northern Quebec, Canada

Mónica Santín;

Mónica Santín

^aEnvironmental Microbial Safety Laboratory, Animal and Natural Resources Institute Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705

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Brent R. Dixon;

Brent R. Dixon

^aEnvironmental Microbial Safety Laboratory, Animal and Natural Resources Institute Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705

^bBureau of Microbial Hazards, Food Directorate, Health Canada, 4th floor West, Banting Research Centre, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2

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Ronald Fayer

^aEnvironmental Microbial Safety Laboratory, Animal and Natural Resources Institute Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705

^cTo whom correspondence should be addressed. [email protected]

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J Parasitol (2005) 91 (3): 712–716.

https://doi.org/10.1645/GE-3438RN

This study reports the molecular characterization of Cryptosporidium spp. isolates identified from intestinal contents of ringed seals (Phoca hispida) from Nunavik (Quebec, Canada). Cryptosporidium spp. fragments of 18S rRNA, HSP-70, and actin loci were amplified by PCR from seal intestinal contents. PCR-positive specimens were sequenced and compared with other Cryptosporidium species and genotypes reported previously. Sequence analysis showed the presence of C. muris and 2 novel genotypes in ringed seals.

American Society of Parasitologists

2005

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Genetic Characterization of Cryptosporidium Isolates From Ringed Seals (Phoca hispida) in Northern Quebec, Canada

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