The levels of expression of Heat shock protein 70 (Hsp 70) in sporozoites of a wild-type parent strain and 2 precocious lines of Eimeria tenella, were compared to investigate the relationship between the heat shock proteins expressed by the parasite and virulence of the strain. Hsp70 expression was analyzed in sporozoites by immunohistochemical techniques, immunoblot, and flow cytometric analyses. One band of 70 kDa was identified and the variation of the Hsp70 expression levels was quantified by optical densitometric analyses. The results showed a significant gradual decrease in the Hsp70 expression in sporozoites of E. tenella as attenuation progressed, suggesting that the Hsp70 expressed in the excysted sporozoites of E. tenella might be involved in parasite pathogenicity. In addition, the cytoplasmic distribution of the Hsp70, which was observed in the entire sporozoites of the wild strain, was reduced to the anterior portion in the precocious lines.
Skip Nav Destination
Article navigation
October 2005
PATHOLOGY|
October 01 2005
DIFFERENCES IN HSP70 EXPRESSION IN THE SPOROZOITES OF THE ORIGINAL STRAIN AND PRECOCIOUS LINES OF EIMERIA TENELLA
E. del Cacho;
E. del Cacho
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Miguel Servet 177, Zaragoza 50013, Spain. [email protected]
Search for other works by this author on:
M. Gallego;
M. Gallego
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Miguel Servet 177, Zaragoza 50013, Spain. [email protected]
Search for other works by this author on:
F. López-Bernad;
F. López-Bernad
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Miguel Servet 177, Zaragoza 50013, Spain. [email protected]
Search for other works by this author on:
J. Quílez;
J. Quílez
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Miguel Servet 177, Zaragoza 50013, Spain. [email protected]
Search for other works by this author on:
C. Sánchez-Acedo
C. Sánchez-Acedo
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Miguel Servet 177, Zaragoza 50013, Spain. [email protected]
Search for other works by this author on:
J Parasitol (2005) 91 (5): 1127–1131.
Citation
E. del Cacho, M. Gallego, F. López-Bernad, J. Quílez, C. Sánchez-Acedo; DIFFERENCES IN HSP70 EXPRESSION IN THE SPOROZOITES OF THE ORIGINAL STRAIN AND PRECOCIOUS LINES OF EIMERIA TENELLA. J Parasitol 1 October 2005; 91 (5): 1127–1131. doi: https://doi.org/10.1645/GE-3464.1
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
MOLECULAR AND NEW MORPHOLOGICAL DATA ON NEMATODE HEDRURIS DRATINI FOUND PARASITIZING PSEUDIS MINUTA (ANURA: HYLIDAE)
Martin M. Montes, Yasmin Croci, Jorge Barneche, Dario Balcazar, German F. Reig Cardarella, Sergio R. Martorelli
CLITELLATE (ANNELIDA: CLITELLATA) PARASITES AND PREDATORS OF NORTH AMERICAN HERPETOFAUNA: CHECKLIST OF SPECIES, IDENTIFICATION KEY, AND A NEW RECORD FOR MEXICO
Manuel De Luna, Roberto García-Barrios, Diane P. Barton, Leonardo García-Vázquez
LOUSY ASSOCIATIONS: SUCKING LICE (PHTHIRAPTERA: ANOPLURA) PARASITIZING RODENTS AND LAGOMORPHS IN THE DESERT NATIONAL WILDLIFE REFUGE, NEVADA
Sara B. Weinstein, John P. Dumbacher, Lance A. Durden
A NEW SPECIES OF ACANTHOBOTHRIUM (CESTODA: ONCHOPROTEOCEPHALIDEA), PARASITE OF THE GIANT ELECTRIC RAY NARCINE ENTEMEDOR JORDAN AND STARKS, 1895 (BATOIDEA: TORPEDINIFORMES) FROM MEXICO
Erick Rodríguez-Ibarra, Berenice Adán-Torres, Fernando Ruiz-Escobar, Gerardo Torres-Carrera
SEROPREVALENCE OF TOXOPLASMA GONDII IN GOATS FROM SOUTHWESTERN MISSISSIPPI, USA
Alexander D. W. Acholonu, Jamela S. Alexander