In trematodes, vitelline precursor proteins are required for eggshell formation. A cDNA clone of Clonorchis sinensis (CsVpB1) was selected from an EST pool, encoding a polypeptide of 245 amino acids. The CsVpB1 polypeptide demonstrated homology with vitelline precursor proteins from trematodes with high sequential identities. In a phylogenic tree, CsVpB1 clustered with trematode VpB proteins. The CsVpB1 polypeptide was found to be rich in tyrosine residues, including putative pre-dihydroxyphenyl alanine (DOPA) residues, involved in cross-linking of the precursor proteins. Mouse immune sera were raised against a recombinant CsVpB1 protein. In adult C. sinensis, CsVpB1 protein was exclusively localized in vitelline follicles. Based on these results, the CsVpB1 cDNA is believed to encode a VpB of C. sinensis.
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1 December 2005
MOLECULAR-CELL BIOLOGY|
December 01 2005
MOLECULAR CLONING AND CHARACTERIZATION OF VITELLINE PRECURSOR PROTEIN B1 FROM CLONORCHIS SINENSIS
Yi Tang;
Yi Tang
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul 156-756, Republic of Korea. hongsj@cau.ac.kr
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Pyo Yun Cho;
Pyo Yun Cho
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul 156-756, Republic of Korea. hongsj@cau.ac.kr
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Bong Soo Kim;
Bong Soo Kim
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul 156-756, Republic of Korea. hongsj@cau.ac.kr
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Sung-Jong Hong
Sung-Jong Hong
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul 156-756, Republic of Korea. hongsj@cau.ac.kr
* To whom correspondence should be addressed
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J Parasitol (2005) 91 (6): 1374–1378.
Citation
Yi Tang, Pyo Yun Cho, Bong Soo Kim, Sung-Jong Hong; MOLECULAR CLONING AND CHARACTERIZATION OF VITELLINE PRECURSOR PROTEIN B1 FROM CLONORCHIS SINENSIS. J Parasitol 1 December 2005; 91 (6): 1374–1378. doi: https://doi.org/10.1645/GE-542R.1
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