Actin is a ubiquitous and highly conserved microfilament protein that is hypothesized to play a mechanical force-generating role in the unusual gliding motility of sporozoan zoites and their active penetration of host cells. We have identified and isolated an actin gene from a Babesia gibsoni cDNA library by random sequencing. The complete nucleotide sequence of the actin gene is 1,243 bp; a single open reading frame encodes a polypeptide of 377 amino acid residues. The deduced amino acid sequence showed a high homology with actins from other species, especially with reported apicomplexan protozoans. The antiserum against recombinant actin expressed in Escherichia coli recognizes a 42-kDa native protein, which is consistent with its expected size. Immunofluorescence and confocal microscopic observation revealed that the protein is diffusely distributed throughout the B. gibsoni parasites.
Isolation and Identification of an Actin Gene From Babesia gibsoni
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Jinlin Zhou, Bing Huang, Hiroshi Suzuki, Kozo Fujisaki, Ikuo Igarashi, Xuenan Xuan; Isolation and Identification of an Actin Gene From Babesia gibsoni. J Parasitol 1 February 2006; 92 (1): 208–210. doi: https://doi.org/10.1645/GE-577R2.1
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