Taeniid cestodes infect humans and livestock, causing considerable morbidity and mortality, as well as economic loss. Substantial progress has been made toward the production of recombinant vaccines against cysticercosis in livestock animals. Further development of these vaccines would be aided if a reliable in vitro test were available to measure host-protective immune responses in vaccinated animals. Here, we describe in vitro oncosphere-killing assays for the quantification of host-protective serum antibodies against Taenia pisiformis, Taenia ovis, Taenia saginata, and Taenia solium in rabbits, sheep, cattle, and pigs, respectively. Activated oncospheres of T. pisiformis, T. ovis, T. saginata, and T. solium were incubated in vitro in culture medium, test serum, and a source of complement, and oncosphere killing was assessed after 10 days of culture. In vitro oncosphere killing reflected the presence of specific antibody, and the oncosphere-killing assay typically indicated immunity to the homologous parasite that had been determined in vivo. This study describes the first reliable oncosphere-killing assays for T. pisiformis, T. ovis, T. saginata, and T. solium. These assays will be used for further research into the optimization of recombinant vaccines against cysticercosis.
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April 2006
Research Article|
April 01 2006
IN VITRO ONCOSPHERE-KILLING ASSAYS TO DETERMINE IMMUNITY TO THE LARVAE OF TAENIA PISIFORMIS, TAENIA OVIS, TAENIA SAGINATA, AND TAENIA SOLIUM
Craig T. Kyngdon;
Craig T. Kyngdon
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
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Charles G. Gauci;
Charles G. Gauci
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
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Rick A. Rolfe;
Rick A. Rolfe
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
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Jeanette C. Velásquez Guzmán;
Jeanette C. Velásquez Guzmán
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
* Laboratorio de Investigacion de Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porras, Lima 31, Peru
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Marilú J. Farfán Salazar;
Marilú J. Farfán Salazar
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
* Laboratorio de Investigacion de Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porras, Lima 31, Peru
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Manuela R. Verástegui Pimentel;
Manuela R. Verástegui Pimentel
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
† Departamento de Microbiologia, Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porras, Lima 31, Peru
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Armando E. Gonzalez;
Armando E. Gonzalez
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
‡ Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Av. Circunvalacion s/n, Salamanca de Monterrico, Lima, Peru
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Hector H. Garcia;
Hector H. Garcia
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
§ Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurologicas, Jr. Ancash 1271, Barrios Altos, Lima 1, Peru
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Robert H. Gilman;
Robert H. Gilman
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
‖ Department of International Health, Johns Hopkins University Bloomberg School of Public Health, 615 N. Wolfe St., Baltimore, Maryland 21205
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Richard A. Strugnell;
Richard A. Strugnell
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
# Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria 3052, Australia
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Marshall W. Lightowlers
Marshall W. Lightowlers
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia. [email protected]
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J Parasitol (2006) 92 (2): 273–281.
Citation
Craig T. Kyngdon, Charles G. Gauci, Rick A. Rolfe, Jeanette C. Velásquez Guzmán, Marilú J. Farfán Salazar, Manuela R. Verástegui Pimentel, Armando E. Gonzalez, Hector H. Garcia, Robert H. Gilman, Richard A. Strugnell, Marshall W. Lightowlers; IN VITRO ONCOSPHERE-KILLING ASSAYS TO DETERMINE IMMUNITY TO THE LARVAE OF TAENIA PISIFORMIS, TAENIA OVIS, TAENIA SAGINATA, AND TAENIA SOLIUM. J Parasitol 1 April 2006; 92 (2): 273–281. doi: https://doi.org/10.1645/GE-619R.1
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