A technique was developed for immunolabeling Cryptosporidium parvum oocysts for subsequent observation by transmission electron microscopy. This method was developed to maintain architectural integrity of the oocyst wall and improve fixation of internal contents. The improved fixation and embedding method permits efficient immunolabeling of both nonexcysted and excysted C. parvum oocysts and may be applicable to other oocyst- and cyst-forming protozoa.
An Improved Electron Microscopic Technique for the Immunolabeling of Cryptosporidium parvum Oocysts
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Mark C. Jenkins, Charles Murphy, James Trout, Ronald Fayer; An Improved Electron Microscopic Technique for the Immunolabeling of Cryptosporidium parvum Oocysts. J Parasitol 1 April 2006; 92 (2): 403–405. doi: https://doi.org/10.1645/GE-3539RN.1
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