Neospora caninum is a world-wide parasite that causes neuromuscular disorders in dogs and bovine abortion. Biological diversity among isolates has been proved in both in vivo and in vitro studies. In contrast, little is known about the genetic diversity of this parasite. Microsatellite sequence analysis constitutes a suitable tool that has been used for the genetic analysis of other apicomplexan parasites. In this report, we describe the identification and analysis of 13 microsatellite loci from N. caninum DNA sequences deposited in public databases, which were evaluated with the use of 9 isolates grown in vitro. One microsatellite was monomorphic, and the remaining 12 loci exhibited 3 to 9 separate alleles. Multilocus analysis showed that each of the 9 isolates investigated here displayed a unique profile and revealed no association between the genetic similarity and host or geographic origin. The multilocus analysis approach described here might nevertheless provide the powerful tool needed to study the genetic complexity of N. caninum and the molecular epidemiology of neosporosis.
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June 2006
Research Article|
June 01 2006
MULTILOCUS MICROSATELLITE ANALYSIS REVEALS EXTENSIVE GENETIC DIVERSITY IN NEOSPORA CANINUM
Javier Regidor-Cerrillo;
Javier Regidor-Cerrillo
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain. luis.ortega@vet.ucm.es
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Susana Pedraza-Díaz;
Susana Pedraza-Díaz
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain. luis.ortega@vet.ucm.es
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Mercedes Gómez-Bautista;
Mercedes Gómez-Bautista
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain. luis.ortega@vet.ucm.es
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Luis M. Ortega-Mora
Luis M. Ortega-Mora
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain. luis.ortega@vet.ucm.es
* To whom correspondence should be addressed
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J Parasitol (2006) 92 (3): 517–524.
Citation
Javier Regidor-Cerrillo, Susana Pedraza-Díaz, Mercedes Gómez-Bautista, Luis M. Ortega-Mora; MULTILOCUS MICROSATELLITE ANALYSIS REVEALS EXTENSIVE GENETIC DIVERSITY IN NEOSPORA CANINUM. J Parasitol 1 June 2006; 92 (3): 517–524. doi: https://doi.org/10.1645/GE-713R.1
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