For some species of 37-collar-spined Echinostoma, their cercariae successfully encyst and develop to metacercariae in vitro. In our study, we cultured Echinostoma cinetorchis cercariae in 12 different media to study the formation of metacercariae. Locke's solution, medium 199, and RPMI 1640 were used as media for culture. RPMI 1640 produced the highest encystment and normal metacercariae development. The osmolality of the media was related to their ability to encyst and develop. The 0.5× media induced higher encystment and normal metacercaria formation than the 1× media. The addition of fetal bovine serum to RPMI 1640 increased the level of encystment and normal metacercariae development. In the mixture of 0.5× RPMI 1640 and 10% fetal bovine serum, encystment was highest, at 96.0%, and the development ratio of normal metacercariae was also the highest, at 91.5%, after 48 hr of cultivation. In a viability test, 7 day- and 14 day-cultured metacercariae were successfully matured to adult worms in experimentally infected rats. These results showed that E. cinetorchis cercariae could be cultured to viable metacercariae in an in vitro culture system and that 0.5× RPMI 1640 plus 10% fetal bovine serum was the most useful medium for cultivation. This culture system can be adapted for additional studies on the E. cinetorchis life cycle, especially to supply large numbers of metacercariae for other studies on this echinostome.
Skip Nav Destination
Article navigation
October 2006
LIFE CYCLES-SURVEY|
October 01 2006
ENCYSTMENT AND METACERCARIAE DEVELOPMENT OF ECHINOSTOMA CINETORCHIS CERCARIAE IN AN IN VITRO CULTURE SYSTEM
Yun-Kyu Park;
Yun-Kyu Park
Department of Parasitology and Inha Research Institute for Medical Sciences, Inha University College of Medicine, Incheon 400-712, Korea; [email protected]
Search for other works by this author on:
Myung-Ki Hwang;
Myung-Ki Hwang
Department of Parasitology and Inha Research Institute for Medical Sciences, Inha University College of Medicine, Incheon 400-712, Korea; [email protected]
Search for other works by this author on:
Pyung-Rim Chung
Pyung-Rim Chung
Department of Parasitology and Inha Research Institute for Medical Sciences, Inha University College of Medicine, Incheon 400-712, Korea; [email protected]
* To whom correspondence should be addressed
Search for other works by this author on:
J Parasitol (2006) 92 (5): 1010–1013.
Citation
Yun-Kyu Park, Myung-Ki Hwang, Pyung-Rim Chung; ENCYSTMENT AND METACERCARIAE DEVELOPMENT OF ECHINOSTOMA CINETORCHIS CERCARIAE IN AN IN VITRO CULTURE SYSTEM. J Parasitol 1 October 2006; 92 (5): 1010–1013. doi: https://doi.org/10.1645/GE-794R.1
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
SEROEPIDEMIOLOGY OF TOXOPLASMA GONDII INFECTION IN DAIRY FARMS FROM AGUASCALIENTES, MEXICO
Carlos Cruz-Vázquez, Enrique Sánchez-Herrera, Irene Vitela-Mendoza, Leticia Medina-Esparza, Erika Rangel-Muñoz, Miguel Ramos-Parra
THE IMPACTS OF ROCK DOVE (COLUMBA LIVIA) PREEN OIL ON WING LOUSE (COLUMBICOLA COLUMBAE) BEHAVIOR AND SURVIVAL
Christopher W. Harbison, Jaimie M. Abraham, Christopher J. Bertola, Joseph C. Celeste, Albert J. Chhay, Keira G. Cohen, Riley A. Mangieri, Danial S. Rehman
VOLATILES IN FECES OF RATS INFECTED WITH THE TAPEWORM HYMENOLEPIS DIMINUTA
Anne C. Jones, Mason C. Martin, Tappey H. Jones, Roger Ramirez-Barrios, Ashleigh B. Smythe
CONTEXT-DEPENDENT EFFECTS OF TREMATODE PARASITES ON SNAIL HOST FEEDING, MOVEMENT, AND BEHAVIOR
Carter E. Watson, William T. Ellis, Kailah Massey, Taylor English, Emlyn J. Resetarits
PREVALENCE OF DIROFILARIA MICROFILARIA IN AMERICAN BLACK BEARS (URSUS AMERICANUS) IN PENNSYLVANIA, 2018–2020
Alia Mazzatosta, Christopher A. Cleveland, Michael J. Yabsley, Ellen Haynes, Hannah S. Tiffin, Erika T. Machtinger, Joshua B. Johnson, Mark Ternent, Emily Carrollo, Justin D. Brown