In this study, we isolated and analyzed a small heat shock protein (HSP) of Ostertagia ostertagi (Oo-HSP18). Oo-hsp18 is encoded by a single-copy gene and the full-length cDNA represents an 18-kDa protein. The expression of Oo-hsp18 is highly stage specific and restricted to the adult stage. The protein is synthesized in a tissue-specific manner and localized in the body muscle layer. The levels of Oo-hsp18 mRNAs are sharply induced by heat shock but not by other stressors such as levamisole and H2O2. A vaccination trial with recombinant Oo-HSP18 failed to protect calves against a challenge infection.
A SMALL HEAT SHOCK PROTEIN OF OSTERTAGIA OSTERTAGI: STAGE-SPECIFIC EXPRESSION, HEAT INDUCIBILITY, AND PROTECTION TRIAL
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Isabel Vercauteren, Veerle De Maere, Jozef Vercruysse, Mieke Stevens, Kris Gevaert, Edwin Claerebout; A SMALL HEAT SHOCK PROTEIN OF OSTERTAGIA OSTERTAGI: STAGE-SPECIFIC EXPRESSION, HEAT INDUCIBILITY, AND PROTECTION TRIAL. J Parasitol 1 December 2006; 92 (6): 1244–1250. doi: https://doi.org/10.1645/GE-871R.1
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