The aim of this study was to examine the feces of free-ranging foxes and coyotes for the presence of Neospora caninum oocysts. Feces were collected from 271 foxes and 185 coyotes in the Canadian province of Prince Edward Island, processed by sucrose flotation, and examined by light microscopy for the presence of coccidian oocysts. In 2 fox and 2 coyote samples, oocysts morphologically and morphometrically similar to oocysts of N. caninum were observed. DNA was extracted from these samples and subjected to nested polymerase chain reaction (PCR) using primers to the N. caninum–specific Nc5 genomic sequence. Through DNA sequencing, alignment of the sequences of at least 3 clones from each isolate to sequences deposited in GenBank revealed 95–99% similarity to the Nc5 sequence of N. caninum. PCR using primers specific for Hammondia heydorni failed to yield an amplification product from these DNA samples.
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December 2006
Research Article|
December 01 2006
NEOSPORA CANINUM–LIKE OOCYSTS OBSERVED IN FECES OF FREE-RANGING RED FOXES (VULPES VULPES) AND COYOTES (CANIS LATRANS)
Wendela Wapenaar
;
Wendela Wapenaar
Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, C1A 4P3, Prince Edward Island, Canada. wwapenaar@gmail.com
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Mark C. Jenkins
;
Mark C. Jenkins
Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, C1A 4P3, Prince Edward Island, Canada. wwapenaar@gmail.com
* Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705
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Ryan M. O'Handley
;
Ryan M. O'Handley
Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, C1A 4P3, Prince Edward Island, Canada. wwapenaar@gmail.com
† Present address: School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch 6150, Western Australia
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Herman W. Barkema
Herman W. Barkema
Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, C1A 4P3, Prince Edward Island, Canada. wwapenaar@gmail.com
‡ Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada
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J Parasitol (2006) 92 (6): 1270–1274.
Citation
Wendela Wapenaar, Mark C. Jenkins, Ryan M. O'Handley, Herman W. Barkema; NEOSPORA CANINUM–LIKE OOCYSTS OBSERVED IN FECES OF FREE-RANGING RED FOXES (VULPES VULPES) AND COYOTES (CANIS LATRANS). J Parasitol 1 December 2006; 92 (6): 1270–1274. doi: https://doi.org/10.1645/GE-913R.1
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