Myoglobins (Mbs), globin proteins, are present in high concentrations in trematodes. In Paragonimus westermani, 2 cDNAs were found to encode Mbs. The first clone, Pwmyo1, codes a total of 149 amino acids with a calculated mass of 16.6 kDa. The second, Pwmyo2, encodes a 146–amino acid protein with a calculated mass of 16.2 kDa. The predicted secondary structures showed the presence of 8 helices, which is the basic characteristic of Mbs. Sequence alignment revealed a high homology with the other trematode Mbs. The 2 clones contained the characteristic tyrosyl residues at helical positions B10 and distal E7, which are substitutions that have been previously shown to contribute to the high oxygen affinity of Mbs. Polyclonal antibodies against the recombinant Mbs were raised with no cross-reactivity observed. Immunolocalization revealed the proteins to be distributed generally throughout the parenchymal tissues, but absent from the tegument and reproductive organs. The cell mass of the eggs of the worm stained positive to Pwmyo2 but not Pwmyo1, suggesting the stage-specific expression of these Mbs.
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February 2007
MOLECULAR-CELL BIOLOGY|
February 01 2007
MOLECULAR CHARACTERIZATION OF TWO MYOGLOBINS OF PARAGONIMUS WESTERMANI
Jefferson V. de Guzman;
Jefferson V. de Guzman
Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Korea. e-mail: [email protected]
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Hak-Sun Yu;
Hak-Sun Yu
Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Korea. e-mail: [email protected]
* Department of Parasitology, Pusan National University, College of Medicine, Pusan 602-739, Korea
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Hae-Jin Jeong;
Hae-Jin Jeong
Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Korea. e-mail: [email protected]
* Department of Parasitology, Pusan National University, College of Medicine, Pusan 602-739, Korea
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Yeon-Chul Hong;
Yeon-Chul Hong
Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Korea. e-mail: [email protected]
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Jin Kim;
Jin Kim
Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Korea. e-mail: [email protected]
† Department of Parasitology, SeoNam University, College of Medicine, Namwon 590-711, Korea
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Hyun-Hee Kong;
Hyun-Hee Kong
Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Korea. e-mail: [email protected]
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Dong-Il Chung
Dong-Il Chung
Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Korea. e-mail: [email protected]
‡ To whom correspondence should be addressed
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J Parasitol (2007) 93 (1): 97–103.
Citation
Jefferson V. de Guzman, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Jin Kim, Hyun-Hee Kong, Dong-Il Chung; MOLECULAR CHARACTERIZATION OF TWO MYOGLOBINS OF PARAGONIMUS WESTERMANI. J Parasitol 1 February 2007; 93 (1): 97–103. doi: https://doi.org/10.1645/GE-846R3.1
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