Microsporidia are obligate intracellular, eukaryotic parasites that are known to infect a variety of invertebrate and vertebrate species and have been reported to include a broad range of host specificities for various cell types. Although it is clear that some species of microsporidia have the ability to disseminate, causing multiorgan infections, it is not understood how dissemination occurs. One hypothesis suggests that mononuclear phagocytes engulf the pathogen and migrate to various organs while the parasite persists and proliferates. This implies that microsporidia have developed methods by which to escape intracellular degradation and can, instead, use the host as a source of nourishment and a vehicle for dissemination. In our study, we investigated the infection kinetics of 2 Encephalitozoon spp. known to cause disseminated disease in humans. Using fluorescence and scanning electron microscopy, it was determined that spore adherence to the host was rapid (3–6 hr), as was the uptake and organization of internal parasitophorous vacuoles (24 hr). Furthermore, replication was shown to occur within macrophages at 72 hr, as measured by the bromodeoxyuridine proliferation assay, and the production of mature spores occurred in host cells at 120 hr. Parasitic replication could be reduced by pretreatment of macrophages with interferon-gamma and bacterial lipopolysaccharide.
Skip Nav Destination
PATHOLOGY| February 01 2008
Kinetics of Encephalitozoon Spp. Infection of Human Macrophages
J Parasitol (2008) 94 (1): 169–175.
Jeffrey Fischer, Diana Tran, Richard Juneau, Hollie Hale-Donze; Kinetics of Encephalitozoon Spp. Infection of Human Macrophages. J Parasitol 1 February 2008; 94 (1): 169–175. doi: https://doi.org/10.1645/GE-1303.1
Download citation file:
Don't already have an account? Register
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your InstitutionSign in via your Institution
Citing articles via
POPULATION STRUCTURE AND BARTONELLA QUINTANA IN HEAD AND BODY LICE IN POKHARA, NEPAL (ANOPLURA: PEDICULIDAE)
Shreekanta S. Poudel, Jefferson A. Vaughan
PALEOEPIDEMIOLOGY OF DIPHYLLOBOTHRIOSIS: CULTURAL AND ENVIRONMENTAL FACTORS AFFECTING ADENOCEPHALUS INTENSITY AND PREVALENCE
Karl J. Reinhard, Bernardo Arriaza, William Alexander Avery, Jane Buikstra, Morgana Camacho, Elizabeth Goodman, John Obafunwa, Bruce Owen, Isabel Teixeira-Santos
TAXONOMIC REASSESSMENT AND MORPHOLOGICAL REDESCRIPTION OF NEMATOSTRIGEA SERPENS ANNULATA (DIGENEA: STRIGEIDAE) FROM OSPREY IN NORTH AMERICA
Bharani Gudla, Sarah A. Orlofske, Sara V. Brant, Vasyl V. Tkach, Shelli Dubay, Lauren Holtz, Tyler J. Achatz
PRESCRIBING PATTERNS AND THE USE OF ANTHELMINTIC DRUGS IN COLOMBIAN PATIENTS: A CROSS-SECTIONAL STUDY
Maria Camila Montes-Montoya, Andrés Gaviria-Mendoza, Maria Mónica Murillo-Muñoz, Jaime Andrés Cardona-Ospina, Jorge Enrique Machado-Alba
MOLECULAR CHARACTERIZATION OF ORNITHOSTRONGYLUS QUADRIRADIATUS ISOLATED FROM A RACING PIGEON (COLUMBA LIVIA DOMESTICA)
Aleksandra Ledwoń, Ines Szotowska, Izabella Dolka, Piotr Szeleszczuk