Cryptosporidium parvum (HNJ-1 strain, genotype 2) merozoites were released from oocysts directly during an incubation and excystation procedure without bleach treatment. They were polymorphic, mostly spindle-shaped; others were bean shaped, actively motile, and underwent division. Merozoites survived for short time–period in an in vitro culture system, but could not be established in a subsequent cultivation effort in RPMI medium.
Observations on Cryptosporidium Life Cycle Stages During Excystation
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Panagiotis Karanis, Akio Kimura, Hideyuki Nagasawa, Ikuo Igarashi, Naoyoshi Suzuki; Observations on Cryptosporidium Life Cycle Stages During Excystation. J Parasitol 1 February 2008; 94 (1): 298–300. doi: https://doi.org/10.1645/GE-1185.1
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