Viable Toxoplasma gondii was isolated by bioassay in mice from tissues of 2 feral cats (Felis domesticus), 2 raccoons (Procyon lotor), a skunk (Mephitis mephitis) trapped in remote locations in Manitoba, Canada, and a black bear (Ursus americanus) from Kuujjuaq, northern Quebec, Canada. Genotyping of these T. gondii isolates using polymorphisms at 10 nuclear markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and an apicoplast marker Apico revealed 4 genotypes. None of the isolates was clonal archetypal Types I, II, and III found in the United States. These results are in contrast with the Type II genotype that is widespread in domestic animals and humans throughout the United States and Europe. This is the first genotyping of T. gondii isolates from this part of North America.
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February 2008
GENETICS-EVOLUTION|
February 01 2008
Isolation and Genetic Characterization of Toxoplasma gondii from Raccoons (Procyon lotor), Cats (Felis domesticus), Striped Skunk (Mephitis mephitis), Black Bear (Ursus americanus), And Cougar (Puma concolor) from Canada
J. P. Dubey;
J. P. Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
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T. Quirk;
T. Quirk
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
* Department of Biology, University of Saskatchewan, Saskatoon, SK, S7N5E2, Canada
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J. A. Pitt;
J. A. Pitt
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
† Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada
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N. Sundar;
N. Sundar
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
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G. V. Velmurugan;
G. V. Velmurugan
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
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O. C H. Kwok;
O. C H. Kwok
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
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D. Leclair;
D. Leclair
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
‡ Nunavik Research Center, Makivik Corporation, Kuujjuaq Quebec JOM 1MO, Canada. Present address: Food Safety Division, Canadian Food Inspection Agency, 159 Cleopatra Drive, Ottawa, ON K1A 0Y9, Canada
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R. Hill;
R. Hill
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
§ Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996-0845
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C. Su
C. Su
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
§ Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996-0845
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J Parasitol (2008) 94 (1): 42–45.
Citation
J. P. Dubey, T. Quirk, J. A. Pitt, N. Sundar, G. V. Velmurugan, O. C H. Kwok, D. Leclair, R. Hill, C. Su; Isolation and Genetic Characterization of Toxoplasma gondii from Raccoons (Procyon lotor), Cats (Felis domesticus), Striped Skunk (Mephitis mephitis), Black Bear (Ursus americanus), And Cougar (Puma concolor) from Canada. J Parasitol 1 February 2008; 94 (1): 42–45. doi: https://doi.org/10.1645/GE-1349.1
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