Viable Toxoplasma gondii was isolated by bioassay in mice from tissues of 2 feral cats (Felis domesticus), 2 raccoons (Procyon lotor), a skunk (Mephitis mephitis) trapped in remote locations in Manitoba, Canada, and a black bear (Ursus americanus) from Kuujjuaq, northern Quebec, Canada. Genotyping of these T. gondii isolates using polymorphisms at 10 nuclear markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and an apicoplast marker Apico revealed 4 genotypes. None of the isolates was clonal archetypal Types I, II, and III found in the United States. These results are in contrast with the Type II genotype that is widespread in domestic animals and humans throughout the United States and Europe. This is the first genotyping of T. gondii isolates from this part of North America.
Skip Nav Destination
Article navigation
February 2008
Research Article|
February 01 2008
Isolation and Genetic Characterization of Toxoplasma gondii from Raccoons (Procyon lotor), Cats (Felis domesticus), Striped Skunk (Mephitis mephitis), Black Bear (Ursus americanus), And Cougar (Puma concolor) from Canada
J. P. Dubey;
J. P. Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
Search for other works by this author on:
T. Quirk;
T. Quirk
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
* Department of Biology, University of Saskatchewan, Saskatoon, SK, S7N5E2, Canada
Search for other works by this author on:
J. A. Pitt;
J. A. Pitt
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
† Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada
Search for other works by this author on:
N. Sundar;
N. Sundar
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
Search for other works by this author on:
G. V. Velmurugan;
G. V. Velmurugan
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
Search for other works by this author on:
O. C H. Kwok;
O. C H. Kwok
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
Search for other works by this author on:
D. Leclair;
D. Leclair
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
‡ Nunavik Research Center, Makivik Corporation, Kuujjuaq Quebec JOM 1MO, Canada. Present address: Food Safety Division, Canadian Food Inspection Agency, 159 Cleopatra Drive, Ottawa, ON K1A 0Y9, Canada
Search for other works by this author on:
R. Hill;
R. Hill
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
§ Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996-0845
Search for other works by this author on:
C. Su
C. Su
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350. jitender.dubey@ars.usda.gov
§ Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996-0845
Search for other works by this author on:
J Parasitol (2008) 94 (1): 42–45.
Citation
J. P. Dubey, T. Quirk, J. A. Pitt, N. Sundar, G. V. Velmurugan, O. C H. Kwok, D. Leclair, R. Hill, C. Su; Isolation and Genetic Characterization of Toxoplasma gondii from Raccoons (Procyon lotor), Cats (Felis domesticus), Striped Skunk (Mephitis mephitis), Black Bear (Ursus americanus), And Cougar (Puma concolor) from Canada. J Parasitol 1 February 2008; 94 (1): 42–45. doi: https://doi.org/10.1645/GE-1349.1
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
GAPEWORM (SYNGAMUS SPP.) PREVALENCE IN WISCONSIN GREATER PRAIRIE CHICKENS (TYMPANUCHUS CUPIDO PINNATUS)
J. A. Shurba, R. A. Cole, M. S. Broadway, C. L. Roderick, J. D. Riddle, S. A. Dubay, S. Hull
OPECHONA CHLOROSCOMBRI AND OPECHONA CORKUMI N. SP. (DIGENEA: LEPOCREADIIDAE) FROM THE NORTHERN GULF OF MEXICO WITH PHYLOGENETIC ANALYSIS BASED ON 28S RDNA
Stephen S. Curran, Steven P. Ksepka, Sergio R. Martorelli, Robin M. Overstreet, Micah B. Warren, Stephen A. Bullard
DIPLOTRIAENA OBTUSA (NEMATODA: DIPLOTRIAENIDAE) FROM BARN SWALLOWS (HIRUNDO RUSTICA) AND CLIFF SWALLOWS (PETROCHELIDON PYRRHONOTA) COLLECTED DURING MORTALITY EVENTS IN THE UPPER MIDWEST, USA
Michelle L. Michalski, Emily Kadolph, Constance L. Roderick, Julia S. Lankton, Rebecca A. Cole
A NEW SPECIES OF EIMERIA (APICOMPLEXA: EIMERIIDAE) FROM THE OLIVE-BACKED POCKET MOUSE, PEROGNATHUS FASCIATUS (RODENTIA: HETEROMYIDAE: PEROGNATHINAE), FROM WYOMING
Delina E. Dority, Zachary P. Roehrs, Chris T. McAllister, R. Scott Seville
A NEW SPECIES OF MYXOBOLUS (CNIDARIA: MYXOSPOREA: MYXOBOLIDAE) FROM THE BLUE SUCKER, CYCLEPTUS ELONGATUS (LESUEUR) (CYPRINIFORMES: CATOSTOMIDAE: CYCLEPTINAE), FROM ARKANSAS
Chris T. McAllister, Ethan T. Woodyard, Justin M. Stilwell, Thomas G. Rosser, Thomas J. Fayton, Alvin J. Camus, Matt J. Griffin, Henry W. Robison
