The immune response of a host infected with Trypanosoma brucei is modulated by trypomastigotes. We examined the changes in cytokine production in T. brucei gambiense (Wellcome strain; WS) infected rats and the influence on production of interleukin (IL)-12 by macrophages. The blood concentration of interferon-γ, tumor necrosis factor–α, and IL-10 increased beginning the second day after infection. However, an increase in IL-12p40 was not observed until 4 days after infection. When spleen macrophages and Kupffer cells harvested from uninfected rats and HS-P cells (a rat macrophagelike cell line) were cocultured with WS, IL-12p40 production did not change. When HS-P cells were cultured with WS, transport of nuclear factor– κB into the nucleus increased. Levels of macrophage colony-stimulating factor (M-CSF) and granulocyte macrophage colony-stimulating factor mRNA in the spleens and livers of WS-infected rats were high in comparison with uninfected rats, suggesting that the WS promotes macrophage proliferation. The level of IL-12p40 mRNA in HS-P cells cocultured with WS increased in response to transfection with a small interfering RNA against M-CSF or addition of anti–M-CSF antibody. These results suggest that the WS inhibits IL-12p40 mRNA production by promoting production of macrophage colony-stimulating factor by macrophages.
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February 2008
Research Article|
February 01 2008
Inhibition of Interleukin-12 Production by Trypanosoma brucei in Rat Macrophages
Kazuhiko Nishimura;
Kazuhiko Nishimura
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. nisimura@vet.osakafu-u.ac.jp
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Shinsuke Sakakibara;
Shinsuke Sakakibara
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. nisimura@vet.osakafu-u.ac.jp
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Kousuke Mitani;
Kousuke Mitani
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. nisimura@vet.osakafu-u.ac.jp
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Jyoji Yamate;
Jyoji Yamate
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. nisimura@vet.osakafu-u.ac.jp
* Laboratory of Infectious Diseases Control, Laboratory of Integrated Structural Biosciences, 1-1, Gakuencho, Nakaku, Sakai, Osaka 599-8531 Japan
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Yoshihiro Ohnishi;
Yoshihiro Ohnishi
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. nisimura@vet.osakafu-u.ac.jp
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Shinji Yamasaki
Shinji Yamasaki
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. nisimura@vet.osakafu-u.ac.jp
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J Parasitol (2008) 94 (1): 99–106.
Citation
Kazuhiko Nishimura, Shinsuke Sakakibara, Kousuke Mitani, Jyoji Yamate, Yoshihiro Ohnishi, Shinji Yamasaki; Inhibition of Interleukin-12 Production by Trypanosoma brucei in Rat Macrophages. J Parasitol 1 February 2008; 94 (1): 99–106. doi: https://doi.org/10.1645/GE-1322.1
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