We previously reported that immunization with intact live cells from schistosomula of Schistosoma japonicum (S.j) partially protected the Kunming strain of mice from challenge infection. In the present work, 2 immune protective experiments were designed to further validate the protective effect induced by this type of vaccine and to optimize the immunization protocol, including the number of inoculations and parasite stages from which immunogenic cells were derived. Three antigens derived from 18-day-old postinfection live (LLC) and dead (DLC) larval worm cells and from dead 42-day-old postinfection adult worm cells (DAC) were used as immunogens. Our results demonstrate that live cells from 18-day-old worms are capable of inducing significant protection in mice using a murine-Sj challenge model as shown by reduction rates of worm recoveries and egg burdens. The development of adult worms was stunted. A Th1-biased immune response was reflected in the protected groups as evidenced by the ratio of IgG2a/IgG1. A 38-kDa polypeptide was recognized by sera from LLC immunized animals. We demonstrate that live parasite cells are a source of novel protective antigens that can be exploited for vaccine development.
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April 2008
Research Article|
April 01 2008
Schistosoma japonicum: Protective Immunity Induced by Schistosomulum-Derived Cells in a Mouse Model
Liting Cai;
Liting Cai
Cell & Molecular Biological Experiment Center and Department of Parasitology Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan 410013, PR China. qingrenz@xysm.net
* These authors contributed equally to this article
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Tiebing Zeng;
Tiebing Zeng
Cell & Molecular Biological Experiment Center and Department of Parasitology Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan 410013, PR China. qingrenz@xysm.net
* These authors contributed equally to this article
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Qingren Zeng;
Qingren Zeng
Cell & Molecular Biological Experiment Center and Department of Parasitology Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan 410013, PR China. qingrenz@xysm.net
† To whom correspondence should be addressed
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Benwen Li;
Benwen Li
Cell & Molecular Biological Experiment Center and Department of Parasitology Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan 410013, PR China. qingrenz@xysm.net
‡ Infectious Diseases Division of School of Medicine, Washington University, St. Louis, Missouri 63110
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Xuechi Lin;
Xuechi Lin
Cell & Molecular Biological Experiment Center and Department of Parasitology Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan 410013, PR China. qingrenz@xysm.net
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Yanfei Gong;
Yanfei Gong
Cell & Molecular Biological Experiment Center and Department of Parasitology Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan 410013, PR China. qingrenz@xysm.net
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Wei Liu;
Wei Liu
Cell & Molecular Biological Experiment Center and Department of Parasitology Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan 410013, PR China. qingrenz@xysm.net
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Zuping Zhang;
Zuping Zhang
Cell & Molecular Biological Experiment Center and Department of Parasitology Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan 410013, PR China. qingrenz@xysm.net
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Shunke Zhang
Shunke Zhang
Cell & Molecular Biological Experiment Center and Department of Parasitology Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan 410013, PR China. qingrenz@xysm.net
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J Parasitol (2008) 94 (2): 395–403.
Citation
Liting Cai, Tiebing Zeng, Qingren Zeng, Benwen Li, Xuechi Lin, Yanfei Gong, Wei Liu, Zuping Zhang, Shunke Zhang; Schistosoma japonicum: Protective Immunity Induced by Schistosomulum-Derived Cells in a Mouse Model. J Parasitol 1 April 2008; 94 (2): 395–403. doi: https://doi.org/10.1645/GE-1315.1
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