The humoral immune response of the tilapia Oreochromis niloticus was evaluated using a direct ELISA. Serum was tested from fish infected with Cichlidogyrus spp. (Monogenea) and from fish injected intraperitoneally with the Cichlidogyrus spp. antigenic extract, i.e., 150 μl of the Cichlidogyrus spp. saline extract diluted in Freund's complete adjuvant (FCA) (1:1) were inoculated intraperitoneally at day 0, followed by 2 dosages of 50 μl of the same Cichlidogyrus spp. saline extract diluted in Freund's incomplete adjuvant (FIA) (1:1) at weeks 2 and 4, respectively. The humoral response was also evaluated by the double immunodiffusion test (DID) and by serum protein and total immunoglobulin (Ig) determinations. The IgM OD values in the hyperimmune fish were significantly higher than in the infected and uninfected fish groups. In the DID test, a precipitation (antigen–antibody) band was observed between the Cichlidogyrus spp. saline extract and hyperimmune sera, but not with the other groups. Increases in serum protein concentration and total Igs were observed in the immunized fish at weeks 2 and 10 postinjection. Results from this study suggest that tilapia is capable of producing an induced humoral immune response against an antigenic extract of Cichlidogyrus spp.
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April 2008
IMMUNOLOGY|
April 01 2008
Humoral Antibody Response of the Tilapia Oreochromis niloticus Against Cichlidogyrus spp. (Monogenea)
Juan José Sandoval-Gío;
Juan José Sandoval-Gío
Laboratorio de Inmunología y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN) Unidad Mérida, Carretera Antigua a Progreso Km, 6 AP 73 Cordemex, 97310 Mérida, Yucatán, México. [email protected]
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Rossanna Rodríguez-Canul;
Rossanna Rodríguez-Canul
Laboratorio de Inmunología y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN) Unidad Mérida, Carretera Antigua a Progreso Km, 6 AP 73 Cordemex, 97310 Mérida, Yucatán, México. [email protected]
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Víctor Manuel Vidal-Martínez
Víctor Manuel Vidal-Martínez
Laboratorio de Inmunología y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN) Unidad Mérida, Carretera Antigua a Progreso Km, 6 AP 73 Cordemex, 97310 Mérida, Yucatán, México. [email protected]
* To whom correspondence should be addressed. Laboratorio de Parasitología, CINVESTAV-IPN, Unidad Mérida, 97310 Mérida, Yucatán, México
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J Parasitol (2008) 94 (2): 404–409.
Citation
Juan José Sandoval-Gío, Rossanna Rodríguez-Canul, Víctor Manuel Vidal-Martínez; Humoral Antibody Response of the Tilapia Oreochromis niloticus Against Cichlidogyrus spp. (Monogenea). J Parasitol 1 April 2008; 94 (2): 404–409. doi: https://doi.org/10.1645/GE-1382.1
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