Perkinsus species are protistan parasites of molluscs. In Chesapeake Bay, Perkinsus marinus, Perkinsus chesapeaki, and Perkinsus andrewsi are sympatric, infecting oysters and clams. Although P. marinus is a pathogen for Crassostrea virginica, it remains unknown whether P. andrewsi and P. chesapeaki are equally pathogenic. Perkinsus species have been reported in C. virginica as far north as Maine, sometimes associated with high prevalence, but low mortality. Thus, we hypothesized that, in addition to P. marinus, Perkinsus species with little or no pathogenicity for C. virginica may be present. Accordingly, we investigated the distribution of Perkinsus species in C. virginica and Mercenaria mercenaria, collected from Maine to Virginia, by applying PCR-based assays specific for P. marinus, P. andrewsi, and a Perkinsus sp. isolated from M. mercenaria. DNA samples of M. mercenaria possessed potent PCR inhibitory activity, which was overcome by the addition of 1 mg/ml BSA and 5% (v/v) DMSO to the PCR reaction mixture. All 3 Perkinsus species were found in both host species throughout the study area. Interestingly, the prevalence of P. marinus in M. mercenaria was significantly lower than in C. virginica, suggesting that M. mercenaria is not an optimal host for P. marinus.
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April 2008
Research Article|
April 01 2008
Assessment of the Northern Distribution Range of Selected Perkinsus Species in Eastern Oysters (Crassostrea virginica) and Hard Clams (Mercenaria mercenaria) with the Use of PCR-Based Detection Assays
Wolf T. Pecher;
Wolf T. Pecher
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202. vasta@umbi.umd.edu
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Mohammad R. Alavi;
Mohammad R. Alavi
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202. vasta@umbi.umd.edu
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Eric J. Schott;
Eric J. Schott
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202. vasta@umbi.umd.edu
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José A. Fernandez-Robledo;
José A. Fernandez-Robledo
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202. vasta@umbi.umd.edu
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Laura Roth;
Laura Roth
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202. vasta@umbi.umd.edu
* L. Roth and S. T. Berg participated in the project as student interns through the Technology Magnet Program, Howard County Public School System, Ellicott City, Maryland, and the Ingenuity Research Practicum, Baltimore Polytechnic Institute, Baltimore, Maryland
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Sean T. Berg;
Sean T. Berg
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202. vasta@umbi.umd.edu
* L. Roth and S. T. Berg participated in the project as student interns through the Technology Magnet Program, Howard County Public School System, Ellicott City, Maryland, and the Ingenuity Research Practicum, Baltimore Polytechnic Institute, Baltimore, Maryland
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Gerardo R. Vasta
Gerardo R. Vasta
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202. vasta@umbi.umd.edu
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J Parasitol (2008) 94 (2): 410–422.
Citation
Wolf T. Pecher, Mohammad R. Alavi, Eric J. Schott, José A. Fernandez-Robledo, Laura Roth, Sean T. Berg, Gerardo R. Vasta; Assessment of the Northern Distribution Range of Selected Perkinsus Species in Eastern Oysters (Crassostrea virginica) and Hard Clams (Mercenaria mercenaria) with the Use of PCR-Based Detection Assays. J Parasitol 1 April 2008; 94 (2): 410–422. doi: https://doi.org/10.1645/GE-1282.1
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