Coccidiosis in chickens is caused by 7 species of Eimeria. Even though coccidiosis is a complex disease that can be caused by any combination of these species, most of the molecular research concerning chicken coccidiosis has been limited to Eimeria tenella. The present study describes the first large-scale analysis of expressed sequence tags (ESTs) generated primarily from second-stage merozoites (and schizonts) of E. acervulina. In total, 1,847 ESTs were sequenced; these represent 1,026 unique sequences. Approximately half of the ESTs encode proteins of unknown function, or hypothetical proteins. Twenty-nine percent of the E. acervulina ESTs share significant sequence identity with sequences in the E. tenella genome. Additionally, EST hits seem to be much different compared with those of E. tenella. One of the differences is the very low number of ESTs that encode putative microneme proteins. This study underlines the potential differences in the molecular aspects of 2 Eimeria species that in the past were thought to be highly similar in nature.
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April 2008
MOLECULAR-CELL BIOLOGY|
April 01 2008
Analysis of Transcripts from Intracellular Stages of Eimeria acervulina Using Expressed Sequence Tags Available to Purchase
K. B. Miska;
K. B. Miska
United States Department of Agriculture, Agricultural Reseach Service, Animal Parasitic Diseases Laboratory, 10300 Baltimore Avenue, Building 1042 BARC-East, Beltsville, Maryland 20705. [email protected]
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R. H. Fetterer;
R. H. Fetterer
United States Department of Agriculture, Agricultural Reseach Service, Animal Parasitic Diseases Laboratory, 10300 Baltimore Avenue, Building 1042 BARC-East, Beltsville, Maryland 20705. [email protected]
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G. H. Rosenberg
G. H. Rosenberg
United States Department of Agriculture, Agricultural Reseach Service, Animal Parasitic Diseases Laboratory, 10300 Baltimore Avenue, Building 1042 BARC-East, Beltsville, Maryland 20705. [email protected]
* Department of Biology, University of New Mexico, Albuquerque, New Mexico 97131
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J Parasitol (2008) 94 (2): 462–466.
Citation
K. B. Miska, R. H. Fetterer, G. H. Rosenberg; Analysis of Transcripts from Intracellular Stages of Eimeria acervulina Using Expressed Sequence Tags. J Parasitol 1 April 2008; 94 (2): 462–466. doi: https://doi.org/10.1645/GE-1186.1
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