Live attenuated coccidiosis vaccines could be used as powerful carriers, expressing exogenous viral and bacterial antigens, to induce protective immunity against pathogenic organisms. We investigated the ability of Eimeria tenella to express an exogenous gene in vitro. Eimeria tenella sporozoites were transfected with the plasmid pH4-2EYFP-Actin3 containing the yellow fluorescent protein gene (yfp) and inoculated into primary chicken kidney cells (PCKCs), followed by incubation at 41 C in a 5% CO2 chamber. Fluorescent sporozoites were observed as early as 15–20 hr post-inoculation (PI). Fluorescence displayed by the expressed YFP protein was visible throughout the schizogony and gametogony stages of the tranfected E. tenella. Fluorescent oocysts were found between 200–327 hr PI. Higher fluorescence intensity was observed in the nucleus than in other compartments of the transfectants, while little or no fluorescence was seen in the refractile globule. The diversity of schizonts, particularly of the first generation, was presented by fluorescent nuclei arranged in different patterns. Our results demonstrated the ability of E. tenella to express an exogenous gene throughout the endogenous development in vitro. Completion of the endogenous development of transfected E. tenella in cell cultures will facilitate the study of foreign antigen expression in Eimeria spp., paving the way for the development of an Eimeria spp. vector vaccine that also carries and delivers other vaccines by oral administration.
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August 2008
RESEARCH NOTES|
August 01 2008
Transfected Eimeria tenella Could Complete Its Endogenous Development In Vitro
T. Y. Shi;
T. Y. Shi
aParasitology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China
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X. Y. Liu;
X. Y. Liu
aParasitology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China
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L. L. Hao;
L. L. Hao
aParasitology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China
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J. D. Li;
J. D. Li
aParasitology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China
bCollege of Agronomy, Ningxia University, Yinchuan, 750021, China
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Abdel Nabi Gh;
Abdel Nabi Gh
aParasitology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China
cDepartment of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, Shambat, Sudan
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M. H. Abdille;
M. H. Abdille
aParasitology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China
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X. Suo‡
X. Suo‡
aParasitology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China
dTo whom correspondence should be addressed. suoxun@cau.edu.cn
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J Parasitol (2008) 94 (4): 978–980.
Citation
T. Y. Shi, X. Y. Liu, L. L. Hao, J. D. Li, Abdel Nabi Gh, M. H. Abdille, X. Suo‡; Transfected Eimeria tenella Could Complete Its Endogenous Development In Vitro. J Parasitol 1 August 2008; 94 (4): 978–980. doi: https://doi.org/10.1645/GE-1412.1
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