Africana dardanelosi n. sp. is described from the intestine of the lizard Hoplocercus spinosus Fitzinger, 1843 collected from southern Amazon, Brazil. Africana dardanelosi is the third species of the genus occurring in the Neotropical realm and is the tenth species assigned to the genus. From other species of Africana from Africa, and especially from the Neotropical species (A. telfordi Bursey and Goldberg, 2002 and A. chabaudi Baker, 1981), A. dardanelosi is easily distinguished by spicule size (2,157–2,403 µm versus 366–458 µm and 644–869 µm, respectively) and by the number of caudal papillae (13 versus 17 and 10, respectively).

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