Gangliosides were isolated from Trypanosoma brucei and analyzed by thin-layer chromatography (TLC) and TLC immunostaining test. Four species of gangliosides, designated as G-1, G-2, G-3, and G-4, were separated by TLC. G-1 ganglioside had the same TLC migration rate as GM3. In contrast, G-2, G-3, and G-4 gangliosides migrated a little slower than GM1, GD1a, and GD1b, respectively. To characterize the molecular species of gangliosides from T. brucei, G-1, G-2, G-3, and G-4 gangliosides were purified and analyzed by TLC immunostaining test with monoclonal antibodies against gangliosides. G-1 ganglioside showed the reactivity to the monoclonal antibody against ganglioside GM3. G-2 was recognized by the anti-GM1 monoclonal antibody. G-3 showed reaction with the monoclonal antibody to GD1a. G-4 had the reactivity to anti-GD1b monoclonal antibody. Using 4 kinds of monoclonal antibodies, we also studied the expression of GM3, GM1, GD1a, and GD1b in T. brucei parasites. GM3, GM1, GD1a, and GD1b were detected on the cell surface of T. brucei. These results suggest that G-1, G-2, G-3, and G-4 gangliosides are GM3 (NeuAcα2-3Galβ1-4Glcβ1-1Cer), GM1 (Galβ1-3GalNAcβ1-4[NeuAcα2-3]Galβ1-4Glcβ1-1Cer), GD1a (NeuAcα2-3Galβ1-3GalNAcβ1-4[NeuAcα2-3]Galβ1-4Glcβ1-1Cer), and GD1b (Galβ1-3GalNAcβ1-4[NeuAcα2-8NeuAcα2- 3]Galβ1-4Glcβ1-1Cer), respectively, and also that they are expressed on the cell surface of T. brucei.
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February 2004
MOLECULAR-CELL BIOLOGY|
February 01 2004
ISOLATION AND CHARACTERIZATION OF GANGLIOSIDES FROM TRYPANOSOMA BRUCEI
Akiko Uemura;
Akiko Uemura
Laboratory of Veterinary Immunology, Division of Veterinary Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. swatarai@vet.osakafu-u.ac.jp
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Shinobu Watarai;
Shinobu Watarai
Laboratory of Veterinary Immunology, Division of Veterinary Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. swatarai@vet.osakafu-u.ac.jp
* To whom correspondence should be addressed
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Yasunori Kushi;
Yasunori Kushi
Laboratory of Veterinary Immunology, Division of Veterinary Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. swatarai@vet.osakafu-u.ac.jp
† Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-0834, Japan
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Takeshi Kasama;
Takeshi Kasama
‡ Instrumental Analysis Research Center for Life Science, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan
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Yoshihiro Ohnishi;
Yoshihiro Ohnishi
Laboratory of Veterinary Immunology, Division of Veterinary Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. swatarai@vet.osakafu-u.ac.jp
§ Laboratory of International of Epidemics, Division of Veterinary Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan
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Hiroshi Kodama
Hiroshi Kodama
Laboratory of Veterinary Immunology, Division of Veterinary Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. swatarai@vet.osakafu-u.ac.jp
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J Parasitol (2004) 90 (1): 123–127.
Citation
Akiko Uemura, Shinobu Watarai, Yasunori Kushi, Takeshi Kasama, Yoshihiro Ohnishi, Hiroshi Kodama; ISOLATION AND CHARACTERIZATION OF GANGLIOSIDES FROM TRYPANOSOMA BRUCEI. J Parasitol 1 February 2004; 90 (1): 123–127. doi: https://doi.org/10.1645/GE-3235
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