Babesia isolates from an elk (Cervus elaphus canadensis) and a caribou (Rangifer tarandus caribou) with fatal infections were compared to Babesia odocoilei (Engeling isolate) from white-tailed deer (Odocoileus virginianus) by experimental infection, serologic, and small subunit ribosomal RNA (SSU rRNA) gene sequence analysis studies. Both the indirect fluorescent antibody test and immunoprecipitation assays demonstrated antigenic variation among the isolates. Experimental infection studies showed no clinical differences among the isolates. Nucleotide sequence analysis showed that the elk and caribou Babesia sp. isolates possessed SSU rRNA genes with identical sequences to that of B. odocoilei. A phylogenetic tree constructed from SSU rRNA gene sequences shows that B. odocoilei is most closely related to Babesia divergens, both of which branch together in the true babesia clade.
PARASITOLOGY|
July 01 2000
ANTIGENIC, PHENOTYPIC AND MOLECULAR CHARACTERIZATION CONFIRMS BABESIA ODOCOILEI ISOLATED FROM THREE CERVIDS
Patricia J. Holman;
Patricia J. Holman
5
1 Department of Veterinary Pathobiology, The Texas Veterinary Medical Center, Texas A&M University, College Station, Texas 77843-4467, USA
5 Corresponding author (e-mail: pholman@cvm.tamu.edu)
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Jena Madeley;
Jena Madeley
1 Department of Veterinary Pathobiology, The Texas Veterinary Medical Center, Texas A&M University, College Station, Texas 77843-4467, USA
2 Current address: B-1B TSSC, Dyess Air Force Base, Abilene, Texas, USA
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T. M. Craig;
T. M. Craig
1 Department of Veterinary Pathobiology, The Texas Veterinary Medical Center, Texas A&M University, College Station, Texas 77843-4467, USA
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B. A. Allsopp;
B. A. Allsopp
3 Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa
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M. T. E. P. Allsopp;
M. T. E. P. Allsopp
3 Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa
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K. R. Petrini;
K. R. Petrini
4 Minnesota Zoological Garden, Apple Valley, Minnesota 55124, USA
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S. D. Waghela;
S. D. Waghela
1 Department of Veterinary Pathobiology, The Texas Veterinary Medical Center, Texas A&M University, College Station, Texas 77843-4467, USA
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G. G. Wagner
G. G. Wagner
1 Department of Veterinary Pathobiology, The Texas Veterinary Medical Center, Texas A&M University, College Station, Texas 77843-4467, USA
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J Wildl Dis (2000) 36 (3): 518–530.
Article history
Received:
April 21 1999
Citation
Patricia J. Holman, Jena Madeley, T. M. Craig, B. A. Allsopp, M. T. E. P. Allsopp, K. R. Petrini, S. D. Waghela, G. G. Wagner; ANTIGENIC, PHENOTYPIC AND MOLECULAR CHARACTERIZATION CONFIRMS BABESIA ODOCOILEI ISOLATED FROM THREE CERVIDS. J Wildl Dis 1 July 2000; 36 (3): 518–530. doi: https://doi.org/10.7589/0090-3558-36.3.518
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