Direct and indirect immunoperoxidase (IP) techniques were evaluated for their potential in identifying infectious pancreatic necrosis (IPN) virus. Both techniques were shown to offer a relatively simple, rapid and efficient means for the specific identification of IPN virus in infected cells. The direct IP method resulted in less nonspecific staining; however, the indirect method was clearly specific and utilized commercially available reagents.

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Author notes

1

Present address: Department of Microbiology, Pennsylvania State University, University Park, Pennsylvania 16802, USA.

© 1978 Wildlife Disease Association
1978