We describe optimization of a peripheral blood mononuclear leukocyte proliferation assay and development of an interleukin-2 receptor (IL-2R) expression assay for bottlenose dolphins (Tursiops truncatus). Peripheral blood mononuclear leukocytes obtained from both Sea World (February 1993) and the Naval Command Control and Ocean Surveillance Center (March 1993) (San Diego, California, USA) were stimulated with the mitogens concanavalin A (ConA) and phytohemagglutinin (PHA) and evaluated for optimum proliferation and IL-2R expression. Based on these optimization assays, standard conditions were established and used to assess immune function in a population of apparently healthy, free-ranging bottlenose dolphins from Sarasota Bay, Florida (USA) in June 1993. A positive correlation was observed between proliferation assays using ConA and PHA as the stimulants. However, IL-2R expression induced by both mitogens differed significantly.
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April 01 1995
DEVELOPMENT OF AN INTERLEUKIN-2 RECEPTOR EXPRESSION ASSAY AND ITS USE IN EVALUATION OF CELLULAR IMMUNE RESPONSES IN BOTTLENOSE DOLPHIN (TURSIOPS TRUNCATUS)
Karen L. Erickson;
Karen L. Erickson
1 Department of Veterinary Pathology, Microbiology, and Immunology, University of California, Davis, California 96616, USA
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Lisa DiMolfetto-Landon;
Lisa DiMolfetto-Landon
1 Department of Veterinary Pathology, Microbiology, and Immunology, University of California, Davis, California 96616, USA
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Randall S. Wells;
Randall S. Wells
2 Chicago Zoological Society, % Mote Marine Laboratories, Sarasota, Florida 34236, USA
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Thomas Reidarson;
Thomas Reidarson
3 Sea World of California Inc., San Diego, California 92109, USA
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Jeffrey L. Stott;
Jeffrey L. Stott
1 Department of Veterinary Pathology, Microbiology, and Immunology, University of California, Davis, California 96616, USA
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David A. Ferrick
David A. Ferrick
4
1 Department of Veterinary Pathology, Microbiology, and Immunology, University of California, Davis, California 96616, USA
4 Address for reprints
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J Wildl Dis (1995) 31 (2): 142–149.
Article history
Received:
October 14 1993
Citation
Karen L. Erickson, Lisa DiMolfetto-Landon, Randall S. Wells, Thomas Reidarson, Jeffrey L. Stott, David A. Ferrick; DEVELOPMENT OF AN INTERLEUKIN-2 RECEPTOR EXPRESSION ASSAY AND ITS USE IN EVALUATION OF CELLULAR IMMUNE RESPONSES IN BOTTLENOSE DOLPHIN (TURSIOPS TRUNCATUS). J Wildl Dis 1 April 1995; 31 (2): 142–149. doi: https://doi.org/10.7589/0090-3558-31.2.142
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