We developed and validated an enzyme immunoassay for immunoreactive pregnanediol-3-glucuronide (iPdG) in feces to monitor reproductive status in desert and Rocky Mountain bighorn sheep (Ovis canadensis). Fecal iPdG concentrations were strongly correlated (r = 0.71) with serum progesterone concentrations in paired fecal and blood samples collected from 34 free-ranging desert bighorn sheep. In bimonthly fecal samples collected from 12 captive ewes, fecal iPdG profiles were similar between desert and Rocky Mountain bighorn sheep and we selected a pregnancy detection cutoff value of iPdG ≥1.8 ng/mg feces. Fecal iPdG concentrations always exceeded this cutoff value when samples were collected from about day 60 of pregnancy to a few days before parturition, but values <1.8 ng/mg (false negatives) were common for samples collected during the first 60 days of gestation. Although we tested a small number of known pregnant and non-pregnant ewes, the accuracy of the assay was 100% when two samples, collected 2 wk apart, were evaluated for any given ewe. Based on these data, this direct enzyme immunoassay for fecal progesterone metabolites has promise as a diagnostic tool to monitor hormone excretion and pregnancy in a free-ranging ungulate species.
PREGNANCY DETECTION IN BIGHORN SHEEP (OVIS CANADENSIS) USING A FECAL-BASED ENZYME IMMUNOASSAY
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Dori L. Borjesson, Walter M. Boyce, Ian A. Gardner, James DeForge, Bill Lasley; PREGNANCY DETECTION IN BIGHORN SHEEP (OVIS CANADENSIS) USING A FECAL-BASED ENZYME IMMUNOASSAY. J Wildl Dis 1 January 1996; 32 (1): 67–74. doi: https://doi.org/10.7589/0090-3558-32.1.67
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