Muskrat (Ondatra zibethicus) immunoglobulin fraction was separated from whole serum by Protein A Sepharose chromatography. In serum electrophoresis, this fraction had a gamma motility; when electrophoresed on a polyacrylamide gel with sodium dodecyl sulfate it resolved into two protein bands of approximately 52 and 25 kilodaltons, respectively These bands were consistent with molecular weights of known heavy and light chains of immunoglobulin G (IgG) in other closely related species. Furthermore, the putative muskrat immunoglobulins had a strong cross-reactivity with mouse IgG1, IgG3, and kappa chain in an enzyme-linked immunosorbent assay. We propose, that the proteins bound to the Protein A Sepharose represent muskrat immunoglobulins of the IgG class.
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October 01 1996
Brief Characterization of Muskrat (Ondatra zibethicus) Immunoglobulin G (IgG) Separated from Serum on Protein A
Joanna D. Borucinska;
Joanna D. Borucinska
1 Northeastern Research Center for Wildlife Diseases, Department of Pathobiology, University of Connecticut, U-89, Storrs, Connecticut 06268, USA
2 Current address: Department of Biology, University of Hartford, 200 Bloomfield Avenue, West Hartford, Connecticut 06117-1559, USA
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Ihsan Ayoub;
Ihsan Ayoub
1 Northeastern Research Center for Wildlife Diseases, Department of Pathobiology, University of Connecticut, U-89, Storrs, Connecticut 06268, USA
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Antonio E. Garmendia
Antonio E. Garmendia
1 Northeastern Research Center for Wildlife Diseases, Department of Pathobiology, University of Connecticut, U-89, Storrs, Connecticut 06268, USA
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J Wildl Dis (1996) 32 (4): 687–690.
Article history
Received:
December 21 1995
Citation
Joanna D. Borucinska, Ihsan Ayoub, Antonio E. Garmendia; Brief Characterization of Muskrat (Ondatra zibethicus) Immunoglobulin G (IgG) Separated from Serum on Protein A. J Wildl Dis 1 October 1996; 32 (4): 687–690. doi: https://doi.org/10.7589/0090-3558-32.4.687
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