Anelpistina californica, new species (Insecta: Zygentoma: Nicoletiidae), from the Peninsula of Baja California, Mexico, is described. We report here the 16S rRNA sequence of this new species. The information in this study will be useful in future studies of nicoletiids from Baja California, especially those that use nicoletiids to calibrate molecular clocks. Baja California populations were separated from mainland continent populations in the late Miocene to Pliocene 13–5 mya when the Gulf of California appeared.

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