A variety of poly(adenosinediphosphoribose) p(ADPR) synthesis inhibitors, structurally related compounds with no inhibitory activity, and agents which reduce radiation-induced G2 arrest, were tested for the concentration dependence of their effect on (a) CHO cell progression to mitosis, (b) the duration of G2 arrest in X-irradiated CHO cells, and (c) [14C]NAD incorporation in permeabilized CHO cells, as a measure of p(ADPR) synthetase activity. Caffeine and nicotinamide uptake by viable cells was also measured. The concentration dependencies for reduction of radiation-induced G2 arrest and for p(ADPR) synthesis inhibition were markedly disparate, although all of the active inhibitors of p(ADPR) synthesis did reduce the duration of radiation-induced G2 arrest to some extent. These data indicate that p(ADPR) synthesis is not a requirement for the induction of G2 arrest by ionizing radiation.
Effect of Poly(Adenosinediphosphoribose) Synthesis Inhibitors and Structurally Related Compounds on Radiation-Induced G2 Arrest
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R. Rowley, J. H. Martin, D. B. Leeper; Effect of Poly(Adenosinediphosphoribose) Synthesis Inhibitors and Structurally Related Compounds on Radiation-Induced G2 Arrest. Radiat Res 1 January 1988; 113 (1): 58–70. doi: https://doi.org/10.2307/3577180
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