Effects of Sodium Butyrate and 3-Aminobenzamide on Survival of Chinese Hamster HA-1 Cells after X Irradiation

HA-1 cells were grown in medium containing 2 mM sodium butyrate and then exposed to graded doses of 250 kVp X rays. After irradiation, some of the butyrate-treated cultures were treated with either 10 or 20 mM 3-aminobenzamide for 2 h at 37°C. The butyrate treatment produced a small degree of radiation sensitization as indicated by an increase in the α parameter using a linear-quadratic description of survival responses. The dose-modifying factor at the 10% survival level$({\rm DMF}_{10})$ was 1.15. Similarly, both 10 and 20 mM 3-aminobenzamide treatments produced concentration-dependent increases in radiosensitization, again as indicated by an increase in the value of the α constant, with${\rm DMF}_{10}$ values of 1.22 and 1.40, respectively. However, the combination of the 2 mM sodium butyrate + 10 mM 3-aminobenzamide treatments produced a supraadditive response in terms of increased cell killing$({\rm DMF}_{10}=1.76)$. We interpret this to mean that 3-aminobenzamide inhibits a sodium butyrate associated increase in poly(ADP-ribose) which then predisposes hyperacetylated chromatin to attack by endogenous nucleases leading to increased cytotoxicity.