Further Studies on the Radiation-Induced Expression of a Tumor-Specific Antigen in Human Cell Hybrids

The neoplastic transformation of human cell hybrids (HeLa × skin fibroblasts) is accompanied by the expression of a cell surface protein for which monoclonal antibodies have been raised. The γ-radiation-induced neoplastic transformation of these cells has been studied where the expression of this cell surface protein, as detected by immunoperoxidase staining, has been used as an end point. The yield of foci of positively staining cells has been shown to increase with increasing time postirradiation at which the assay is done and decrease with increasing density of viable cells plated postirradiation. The time of plating postirradiation is also an important parameter with transformation frequencies increasing over the first 6 h of postirradiation holding at confluence, followed by a gradual decrease.