Repair of potentially lethal damage (PLD) was investigated in a γ-ray-sensitive Chinese hamster cell mutant, XR-1, and its parent by comparing survival of plateau-phase cells plated immediately after irradiation with cells plated after a delay. Previous work indicated that XR-1 cells are deficient in repair of double-strand DNA breaks and are γ-ray sensitive in G1 but have near normal sensitivity and repair capacity in late S phase. At irradiation doses from 0 to 1.0 Gy (100 to 10% survival), the delayed- and immediate-plating survival curves of XR-1 cells were identical; however, at doses greater than 1.0 Gy a significant increase in survival was observed when plating was delayed (PLD repair), approaching a 20-fold increase at 8 Gy. Elimination of S-phase cells by [3 H]thymidine suicide dramatically increased γ-ray sensitivity of plateau-phase XR-1 mutant cells and reduced by 600-fold the number of cells capable of PLD repair after a 6-Gy dose. In contrast, elimination of S-phase cells in plateau-phase parental cells did not alter PLD repair. These results suggest that the majority of PLD repair observed in plateau-phase XR-1 cells occurs in S-phase cells while G1 cells perform little PLD repair. In contrast, G1 cells account for the majority of PLD repair in plateau-phase parental cells. Thus, in the XR-1 mutant, a cell's ability to repair PLD seems to depend upon the stage of the cell cycle at which the irradiation is delivered. A possible explanation for these findings is discussed.
Cell-Cycle-Dependent Repair of Potentially Lethal Damage in the XR-1 γ-Ray-Sensitive Chinese Hamster Ovary Cell
- Views Icon Views
- Share Icon Share
- Search Site
Thomas D. Stamato, Arthur Dipatri, Amato Giaccia; Cell-Cycle-Dependent Repair of Potentially Lethal Damage in the XR-1 γ-Ray-Sensitive Chinese Hamster Ovary Cell. Radiat Res 1 August 1988; 115 (2): 325–333. doi: https://doi.org/10.2307/3577168
Download citation file: