The effects of pentobarbital anesthesia on the energy metabolism of FSall and MCaIV foot tumors in mice were studied by 31P MRS. Using an 8.5 T spectrometer, in vivo spectra were obtained in 15 animals before and after pentobarbital anesthesia (0.05 mg/g ip). The average phosphocreatine/inorganic phosphate ratios PCr/Pi with and without pentobarbital were similar for both tumor histologies. Effects on individual tumors, however, were greater than 20% in 9/15 animals and greater than 50% in 6/15 animals. Pentobarbital anesthesia increased the variability of tumor intracellular pH, and the phosphomonoester/nucleotide triphosphate (PME/NTP) and nucleotide triphosphate/inorganic phosphate ratios \rm NTP/Pi. When examining the average in a cohort, pentobarbital anesthesia had no significant effect on the PCr/PPi, PME/NTP, NTP/PPi ratios or the pH. However, ≃50% of individual tumors do have significant changes in these parameters. The anesthesia-induced variability of tumor energy metabolism may explain the decrease in TCD50 observed in previous studies using multifraction radiation.
Effects of Pentobarbital Anesthesia on the Energy Metabolism of Murine Tumors Studied by in Vivo 31P Nuclear Magnetic Resonance Spectroscopy
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Paul Okunieff, Ernst Rummeny, Peter Vaupel, Steven Skates, Christopher Willett, Leo J. Neuringer, Herman D. Suit; Effects of Pentobarbital Anesthesia on the Energy Metabolism of Murine Tumors Studied by in Vivo 31P Nuclear Magnetic Resonance Spectroscopy. Radiat Res 1 August 1988; 115 (2): 361–372. doi: https://doi.org/10.2307/3577171
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