When poly(dA), poly(dA-dT), and salmon testis DNA were γ-irradiated under nitrogen, the major deoxyadenosine damage product (excluding liberated adenine) was identified as the α-anomer of deoxyadenosine. The yields of α-deoxyadenosine from poly(dA), poly(dA-dT), and salmon testis DNA irradiated with a dose of 500 Gy under anoxic conditions were 1.5, 1.3, and 1.3%, respectively. No α-deoxyadenosine was detected after irradiation under oxic conditions. The presence of nucleotides with the α-configuration at the anomeric carbon atom in the DNA chain may have a significant effect on its tertiary structure and possibly modify its biological activity.
Formation of α-Deoxyadenosine in Polydeoxynucleotides Exposed to Ionizing Radiation under Anoxic Conditions
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Krystyna B. Lesiak, Kenneth T. Wheeler; Formation of α-Deoxyadenosine in Polydeoxynucleotides Exposed to Ionizing Radiation under Anoxic Conditions. Radiat Res 1 March 1990; 121 (3): 328–337. doi: https://doi.org/10.2307/3577784
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