Synchronous Chinese hamster ovary cells in early S phase were obtained by selecting mitotic cells, accumulating them at the G1/ S border by incubating them in aphidicolin for 12 h, and then incubating them for 2 h after releasing them from the aphidicolin block. To determine if thermotolerance could be induced, the cells were heated at 43°C for 20 min in early S phase, incubated for 160 min, and then heated a second time at 43°C for different durations (30-100 min). For the control, nontolerant population, the cells in early S phase were incubated for 50 min and then heated once at 43°C for different durations (20-60 min). Flow cytometric analysis indicated that the population receiving the second heat dose was in the same part of S phase as the population receiving the single heat dose. A comparison of the heat response for the two populations indicated that heating during early S phase induced thermotolerance for both cell killing and chromosomal aberrations; i.e., for 10% survival, which corresponded to 10% of the cells being cytologically normal, the thermal dose was twofold greater in the thermotolerant cells than in the control, nontolerant cells. Furthermore, this thermotolerance developed during S phase. These observations support the hypothesis that heating during S phase kills cells primarily by inducing chromosomal aberrations.
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Research Article| May 01 1990
Thermal Tolerance during S Phase for Cell Killing and Chromosomal Aberrations
Radiat Res (1990) 122 (2): 193–196.
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XiLian Li, R. S. L. Wong, W. C. Dewey; Thermal Tolerance during S Phase for Cell Killing and Chromosomal Aberrations. Radiat Res 1 May 1990; 122 (2): 193–196. doi: https://doi.org/10.2307/3577606
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