The Effect of Light Fluence Rate in Photodynamic Therapy of Normal Rat Brain

This paper reports the effect of incident light fluence rate on the depth to which necrotic lesions are produced by photodynamic therapy (PDT) in the brains of normal Fisher rats. The rats were injected intraperitoneally with Photofrin$(12.5\ {\rm mg}\ {\rm kg}^{-1})$ 48 h prior to PDT with a fixed incident fluence of$35\ {\rm J}\ {\rm cm}^{-2}$. The treatment was performed at 10, 50, 100, and 200 mW cm^-2 and also in a periodic manner (30 s "on" at$100\ {\rm mW}\ {\rm cm}^{-2}$, 30 s "off"). The depth to which necrosis occurred was determined 24 h after treatment by microscopic examination of tissue sections. No differences were found in the depth to which necrosis was produced by any of the five irradiation schedules. This finding is discussed in the context of other published dose-rate experiments.