WR-1065, the free-thiol form of WR-2721, has radioprotective effects in various biological systems. We measured the efficiency of WR-1065 in modifying the induction of chromosome aberrations by X rays in human lymphocytes. G0 lymphocytes were incubated for 30 min in medium containing 1-12 mM WR-1065, exposed to 0 or 3.1 Gy 220-kV X rays, washed, and cultured for evaluations of chromosome aberrations and micronuclei (MN). Neither proliferation kinetics nor baseline frequencies of aberrations or MN were affected in nonirradiated cultures incubated in WR-1065 for up to 45 min. Radiation-induced chromosome aberrations and MN varied inversely as a logarithmic function of thiol concentration. At extracellular concentrations of 8-12 mM, WR-1065 protected against >85% of X-ray-induced chromosome damage as measured by either cytogenetic end point. WR-1065 is more efficient in modulating X-ray-induced chromosome aberrations than dimethyl sulfoxide, which provides protection by scavenging OH radicals. Our data suggest that mechanisms in addition to OH radical scavenging are involved in radioprotection by WR-1065.
Concentration-Dependent Protection against X-Ray-Induced Chromosome Aberrations in Human Lymphocytes by the Aminothiol WR-1065
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L. Gayle Littlefield, Eugene E. Joiner, Shirley P. Colyer, Fatma Sallam, Edward L. Frome; Concentration-Dependent Protection against X-Ray-Induced Chromosome Aberrations in Human Lymphocytes by the Aminothiol WR-1065. Radiat Res 1 January 1993; 133 (1): 88–93. doi: https://doi.org/10.2307/3578261
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