The induction and closure of double-strand breaks produced by X rays were measured in the replicating DNA of HeLa S3 cells using the techniques of neutral (pH 7.2) filter elution and pulsed-field agarose gel electrophoresis. In whole cell DNA the apparent yield of double-strand breaks in pulse-labeled DNA was approximately half that observed in bulk DNA as estimated by both techniques. In contrast, when nuclear DNA was reduced to sub-replicon-cluster lengths prior to irradiation, the yield of radiation-induced double-strand breaks was the same in both replicating and bulk DNA. During incubation of pulse-labeled whole cells at 37°C, the sensitivity of pulse-labeled DNA to strand break induction approached that observed in bulk DNA with a half-time of approximately 105 min. The results indicate that double-strand breaks are produced at a similar frequency per DNA mass in both replicating and bulk DNA. The structure of replicating DNA obscures length reduction in whole cell DNA when estimated by either filter elution or gel electrophoresis. Closure of double-strand breaks proceeded at a similar rate in both replicating and bulk DNA.
Skip Nav Destination
Research Article| June 01 1993
Induction and Repair of Double-Strand Breaks in the Replicating DNA of HeLa Cells
Radiat Res (1993) 134 (3): 337–342.
- Views Icon Views
- Share Icon Share
- Search Site
Raymond L. Warters, Bradley W. Lyons; Induction and Repair of Double-Strand Breaks in the Replicating DNA of HeLa Cells. Radiat Res 1 June 1993; 134 (3): 337–342. doi: https://doi.org/10.2307/3578194
Download citation file:
Citing articles via
Commonalities Between COVID-19 and Radiation Injury
Carmen I. Rios, David R. Cassatt, Brynn A. Hollingsworth, Merriline M. Satyamitra, Yeabsera S. Tadesse, Lanyn P. Taliaferro, Thomas A. Winters, Andrea L. DiCarlo
Low-Dose Radiation Therapy (LDRT) for COVID-19: Benefits or Risks?
Pataje G. Prasanna, Gayle E. Woloschak, Andrea L. DiCarlo, Jeffrey C. Buchsbaum, Dörthe Schaue, Arnab Chakravarti, Francis A. Cucinotta, Silvia C. Formenti, Chandan Guha, Dale J. Hu, Mohammad K. Khan, David G. Kirsch, Sunil Krishnan, Wolfgang W. Leitner, Brian Marples, William McBride, Minesh P. Mehta, Shahin Rafii, Elad Sharon, Julie M. Sullivan, Ralph R. Weichselbaum, Mansoor M. Ahmed, Bhadrasain Vikram, C. Norman Coleman, Kathryn D. Held
Germicidal Efficacy and Mammalian Skin Safety of 222-nm UV Light
Manuela Buonanno, Brian Ponnaiya, David Welch, Milda Stanislauskas, Gerhard Randers-Pehrson, Lubomir Smilenov, Franklin D. Lowy, David M. Owens, David J. Brenner