Two aneuploid cell lines which differ in their inherent sensitivity to ionizing radiation and chemotherapeutic agents were established concurrently from a single tumor specimen obtained from a patient with glioblastoma. M059J cells are approximately 30-fold more sensitive to radiation than are M059K cells (surviving fractions at 2 Gy were 0.02 and 0.64, respectively). This relative difference in radiation sensitivity has remained a stable feature of the cell lines during 2 years in continuous culture. In addition, cells of the M059J line are more sensitive than those of the M059K line to the cytotoxic effects of bleomycin, N,N-bis(2-chloroethyl)-N-nitrosourea, and nitrogen mustard. These cell lines may prove to provide a useful model system for evaluating the cellular and molecular processes which confer resistance or sensitivity in cancer treatment.
Isolation of Two Cell Lines from a Human Malignant Glioma Specimen Differing in Sensitivity to Radiation and Chemotherapeutic Drugs
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M. J. Allalunis-Turner, G. M. Barron, R. S. Day,, K. D. Dobler, R. Mirzayans; Isolation of Two Cell Lines from a Human Malignant Glioma Specimen Differing in Sensitivity to Radiation and Chemotherapeutic Drugs. Radiat Res 1 June 1993; 134 (3): 349–354. doi: https://doi.org/10.2307/3578196
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