The induction of hsp-70 by γ irradiation was studied in Chinese hamster ovary (CHO) cells. Synthesis of hsp-70 was assessed by SDS-PAGE of radiolabeled proteins while levels of hsp-70 mRNA were estimated by Northern blot analysis of total RNA. SDS-PAGE analysis indicated that the synthesis of hsp-70 was enhanced in cells exposed to 400 or 1000 Gy. Northern blot analysis of RNA from cells exposed to 1000 Gy indicated a rapid increase in mRNA capable of hybridizing to an hsp-70 oligonucleotide probe. These experiments represent the first report demonstrating that ionizing radiation can increase hsp-70 mRNA.
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Research Article| July 01 1993
Gamma Irradiation Increases hsp-70 in Chinese Hamster Ovary Cells
Elaine Sierra-Rivera ;
Gerard J. Voorhees ;
Radiat Res (1993) 135 (1): 40–45.
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Elaine Sierra-Rivera, Gerard J. Voorhees, Michael L. Freeman; Gamma Irradiation Increases hsp-70 in Chinese Hamster Ovary Cells. Radiat Res 1 July 1993; 135 (1): 40–45. doi: https://doi.org/10.2307/3578394
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