The cytotoxic and mutagenic effects of radon and its progeny were compared in murine lymphoblast L5178Y-R16 cells after exposure at three institutions. The cells were exposed to222 Rn at Case Western Reserve University (CWRU) and Pacific Northwest Laboratories (PNL) and to212 Bi, a decay product of220 Rn, at the University of Chicago (UC). The dose to the cell nucleus was calculated using a dosimetric model which addressed both the contribution of the dose from the radioactivity in the medium and that associated with the cells. The dose-response curves for cell survival showed$D_{0}\text{'}{\rm s}$ of 0.30 Gy at CWRU, 0.20 Gy at PNL, 0.37 Gy for chelated212 Bi, and 0.13 Gy for unchelated212 Bi. Induced mutant frequencies at the thymidine kinase locus at the 37% survival level were$1470\times 10^{-6}$ at CWRU, 1518 at PNL, and$2414\times 10^{-6}$ at UC using combined results for chelated and unchelated212 Bi. The variation between institutions was greater than obtained in a previous interlaboratory comparison of the effects of radon on CHO cells. Since less radioactivity was associated with CHO cells than L5178Y cells, we have concluded that the variation between institutions in the case of L5178Y cells is caused by the differences in cell-associated radioactivity and errors related to the measurement of this parameter.
Skip Nav Destination
Close
Article navigation
October 1993
Research Article|
October 01 1993
Interlaboratory Comparison of the Effects of Radon on L5178Y Cells: Dose Contribution of Radon Daughter Association with Cells
Radiat Res (1993) 136 (1): 48–56.
Citation
H. H. Evans, J. Mencl, G. Bakale, P. S. Rao, R. F. Jostes, T. E. Hui, F. T. Cross, J. L. Schwartz; Interlaboratory Comparison of the Effects of Radon on L5178Y Cells: Dose Contribution of Radon Daughter Association with Cells. Radiat Res 1 October 1993; 136 (1): 48–56. doi: https://doi.org/10.2307/3578639
Download citation file:
Close
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
Commonalities Between COVID-19 and Radiation Injury
Carmen I. Rios, David R. Cassatt, Brynn A. Hollingsworth, Merriline M. Satyamitra, Yeabsera S. Tadesse, Lanyn P. Taliaferro, Thomas A. Winters, Andrea L. DiCarlo
Low-Dose Radiation Therapy (LDRT) for COVID-19: Benefits or Risks?
Pataje G. Prasanna, Gayle E. Woloschak, Andrea L. DiCarlo, Jeffrey C. Buchsbaum, Dörthe Schaue, Arnab Chakravarti, Francis A. Cucinotta, Silvia C. Formenti, Chandan Guha, Dale J. Hu, Mohammad K. Khan, David G. Kirsch, Sunil Krishnan, Wolfgang W. Leitner, Brian Marples, William McBride, Minesh P. Mehta, Shahin Rafii, Elad Sharon, Julie M. Sullivan, Ralph R. Weichselbaum, Mansoor M. Ahmed, Bhadrasain Vikram, C. Norman Coleman, Kathryn D. Held
Germicidal Efficacy and Mammalian Skin Safety of 222-nm UV Light
Manuela Buonanno, Brian Ponnaiya, David Welch, Milda Stanislauskas, Gerhard Randers-Pehrson, Lubomir Smilenov, Franklin D. Lowy, David M. Owens, David J. Brenner