Fluorescence in situ hybridization procedures were used to examine the influence of chromosome locus on the frequency and type of chromosome aberrations induced by60 Co γ rays in the human lymphoblastoid cell line TK6. Aberrations involving the X chromosome were compared to those involving the similarly sized autosome chromosome 7. When corrected for DNA content, acentric fragments were induced with equal frequency in the X and 7 chromosomes. Dose-dependent increases in chromosomal interchanges involving chromosome 7 were noted, and the frequencies of balanced translocations and dicentrics produced were approximately equal. Chromosome interchanges involving the X chromosome were rare and showed no apparent dose dependence. Thus, while chromosomes 7 and X are equally sensitive to the induction of chromosome breaks, the X chromosome is much less likely to interact with autosomes than chromosome 7. The noninvolvement of the X chromosome in translocations with autosomes may reflect a more peripheral and separate location for the X chromosome in the mammalian nucleus.
Noninvolvement of the X Chromosome in Radiation-Induced Chromosome Translocations in the Human Lymphoblastoid Cell Line TK6
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Robert Jordan, Jeffrey L. Schwartz; Noninvolvement of the X Chromosome in Radiation-Induced Chromosome Translocations in the Human Lymphoblastoid Cell Line TK6. Radiat Res 1 March 1994; 137 (3): 290–294. doi: https://doi.org/10.2307/3578701
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