A polymerase chain reaction (PCR) technique has been developed to detect deletions in the mouse retinoblastoma gene using histological sections from radiation-induced and spontaneous tumors as the DNA source. Six mouse Rb gene exon fragments were amplified in a 40-cycle, 3-temperature PCR protocol. The absence of any of these fragments relative to control PCR products on a Southern blot indicated a deletion of that portion of the mouse Rb gene. Tumors chosen for analysis were lung adenocarcinomas that were judged to be the cause of death. Spontaneous tumors as well as those from irradiated mice (5.69 Gy60 Co γ rays or 0.6 Gy JANUS neutrons, which have been found to have approximately equal radiobiological effectiveness) were analyzed for mouse Rb deletions. Tumors in 6 neutron-irradiated mice had no mouse Rb deletions. However, 1 of 6 tumors from γ-irradiated mice (17%) and 6 of 18 spontaneous tumors from unirradiated mice (33%) showed a deletion in one or both mouse Rb alleles. All deletions detected were in the 5′ region of the mouse Rb gene.
Detection of Retinoblastoma Gene Deletions in Spontaneous and Radiation-Induced Mouse Lung Adenocarcinomas by Polymerase Chain Reaction
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Mark E. Churchill, M. Anne Gemmell, Gayle E. Woloschak; Detection of Retinoblastoma Gene Deletions in Spontaneous and Radiation-Induced Mouse Lung Adenocarcinomas by Polymerase Chain Reaction. Radiat Res 1 March 1994; 137 (3): 310–316. doi: https://doi.org/10.2307/3578704
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