Recent interest has focused on the identification of molecular genetic mechanisms in multistep neoplastic transformation. In vitro exposure of simian virus 40 (SV40)-immortalized human uroepithelial cells (SV-HUC) that are environmentally relevant to bladder carcinogens has been shown to produce tumorigenic transformation, as assessed by the ability of cells exposed to a carcinogen to form xenograph tumors with heterogeneous cancer phenotypes ranging from very aggressive, invasive high-grade carcinomas to superficial low-grade indolent tumors. In addition, exposure of a low-grade indolent tumor generated in the SV-HUC system, MC-T11, to the same carcinogens results in neoplastic progression as assessed by the production of highgrade aggressive cancers. In the present study, we show neoplastic progression of MC-T11 after in vitro exposure to a single dose of 6 Gy X rays. In addition, we show that the chromosome deletions, including losses of 4q, 11p, 13q and 18, observed in these radiation-induced tumors are similar to those observed in carcinogen-induced tumors, thus supporting the hypothesis that the experimental cell system, not the transforming agent, dictates the genetic losses required for tumorigenic transformation and progression.
In Vitro Radiation-Induced Neoplastic Progression of Low-Grade Uroepithelial Tumors
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Simonetta Pazzaglia, Xiao-Rong Chen, Carla B. Aamodt, Shi-Qi Wu, Chinghai Kao, Kennedy W. Gilchrist, Ryoichi Oyasu, Catherine A. Reznikoff, Mark A. Ritter; In Vitro Radiation-Induced Neoplastic Progression of Low-Grade Uroepithelial Tumors. Radiat Res 1 April 1994; 138 (1): 86–92. doi: https://doi.org/10.2307/3578850
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