We studied radiation-induced apoptosis in rat embryo cells (REC) and in REC transfected with a c-myc oncogene [REC:myc(ch1)] or with a c-Ha-ras oncogene [REC:ras(ch1)], or both [REC:myc+ras(ch1)]. Apoptosis, evaluated in terms of altered morphology, dye exclusion and DNA fragmentation, was evident in all the irradiated cells. The development of apoptosis with time was initially rapid and then gradually saturated at >36 h after irradiation. Radiation-induced apoptosis in REC:ras(ch1) was mildly reduced, but that in REC:ras(ch1) and REC:myc+ras(ch1) was significantly increased, relative to that in REC. The percentage of REC:myc(ch1) and REC:myc+ras(ch1) cells undergoing apoptosis increased rapidly at low doses to about 40% at 5 Gy irradiation and reached a plateau at high doses (of about 60% at >15 Gy). In contrast, REC and REC:ras(ch1) were much less responsive, with a maximum of about 15 and 7%, respectively.
Skip Nav Destination
Research Article| September 01 1994
Transfected c-myc and c-Ha-ras Modulate Radiation-Induced Apoptosis in Rat Embryo Cells
Chang Hu Chen ;
Radiat Res (1994) 139 (3): 307–315.
- Views Icon Views
- Share Icon Share
- Search Site
Chang Hu Chen, Jie Zhang, C. Clifton Ling; Transfected c-myc and c-Ha-ras Modulate Radiation-Induced Apoptosis in Rat Embryo Cells. Radiat Res 1 September 1994; 139 (3): 307–315. doi: https://doi.org/10.2307/3578828
Download citation file:
Citing articles via
Commonalities Between COVID-19 and Radiation Injury
Carmen I. Rios, David R. Cassatt, Brynn A. Hollingsworth, Merriline M. Satyamitra, Yeabsera S. Tadesse, Lanyn P. Taliaferro, Thomas A. Winters, Andrea L. DiCarlo
Monte Carlo Simulation of SARS-CoV-2 Radiation-Induced Inactivation for Vaccine Development
Ziad Francis, Sebastien Incerti, Sara A. Zein, Nathanael Lampe, Carlos A. Guzman, Marco Durante
Low-Dose Radiation Therapy (LDRT) for COVID-19: Benefits or Risks?
Pataje G. Prasanna, Gayle E. Woloschak, Andrea L. DiCarlo, Jeffrey C. Buchsbaum, Dörthe Schaue, Arnab Chakravarti, Francis A. Cucinotta, Silvia C. Formenti, Chandan Guha, Dale J. Hu, Mohammad K. Khan, David G. Kirsch, Sunil Krishnan, Wolfgang W. Leitner, Brian Marples, William McBride, Minesh P. Mehta, Shahin Rafii, Elad Sharon, Julie M. Sullivan, Ralph R. Weichselbaum, Mansoor M. Ahmed, Bhadrasain Vikram, C. Norman Coleman, Kathryn D. Held
Studies of the Mortality of Atomic Bomb Survivors, Report 14, 1950–2003: An Overview of Cancer and Noncancer Diseases
Kotaro Ozasa, Yukiko Shimizu, Akihiko Suyama, Fumiyoshi Kasagi, Midori Soda, Eric J. Grant, Ritsu Sakata, Hiromi Sugiyama, Kazunori Kodama
Fluoroscopy X-Ray Organ-Specific Dosimetry System (FLUXOR) for Estimation of Organ Doses and Their Uncertainties in the Canadian Fluoroscopy Cohort Study
A. Iulian Apostoaei, Brian A. Thomas, F. Owen Hoffman, David C. Kocher, Kathleen M. Thiessen, David Borrego, Choonsik Lee, Steven L. Simon, Lydia B. Zablotska